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Titolo:
Annexin 2 has an essential role in actin-based macropinocytic rocketing
Autore:
Merrifield, CJ; Rescher, U; Almers, W; Proust, J; Gerke, V; Sechi, AS; Moss, SE;
Indirizzi:
Univ Coll London, Inst Ophthalmol, Div Cell Biol, London EC1V 9EL, EnglandUniv Coll London London England EC1V 9EL Biol, London EC1V 9EL, England Oregon Hlth Sci Univ, Vollum Inst, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 m Inst, Portland, OR 97201 USA Univ Munster, Zentrum Molekularbiol Entzundung, Inst Med Biochem, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 d Biochem, D-48149 Munster, Germany Univ Coll London, Dept Physiol, London WC1E 6BT, England Univ Coll LondonLondon England WC1E 6BT ysiol, London WC1E 6BT, England Gesell Biotechnol Forsch GmbH, Dept Cell Biol, D-38124 Braunschweig, Germany Gesell Biotechnol Forsch GmbH Braunschweig Germany D-38124 weig, Germany
Titolo Testata:
CURRENT BIOLOGY
fascicolo: 14, volume: 11, anno: 2001,
pagine: 1136 - 1141
SICI:
0960-9822(20010724)11:14<1136:A2HAER>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LISTERIA-MONOCYTOGENES; CHROMAFFIN CELLS; EARLY ENDOSOMES; MOTILITY; POLYMERIZATION; EXOCYTOSIS; MEMBRANE; CYTOSKELETON; ASSOCIATION; GRANULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Moss, SE Univ Coll London, Inst Ophthalmol, Div Cell Biol, Bath St, LondonEC1V 9EL, England Univ Coll London Bath St London England EC1V 9EL 1V 9EL, England
Citazione:
C.J. Merrifield et al., "Annexin 2 has an essential role in actin-based macropinocytic rocketing", CURR BIOL, 11(14), 2001, pp. 1136-1141

Abstract

Annexin 2 is a Ca (2+) binding protein that binds to and aggregates secretory vesicles at physiological Ca 2+ levels [1] and that also associates Ca2 independently with early endosomes; [2, 3]. These properties suggest roles in both exocytosis and endocytosis, but little is known of the dynamics of Annexin 2 distribution in live cells during these processes. We have usedevanescent field microscopy to image Annexin 2-GFP in live, secreting rat basophilic leukemia cells and in cells performing pinocytosis. Although we found no evidence of Annexin 2 involvement in exocytosis, we observed an enrichment of Annexin 2-GFP in actin tails propeling macropinosomes. The association of Annexin 2-GFP with rocketing macropinosomes was specific becauseAnnexin 2-GFP was absent from the actin tails of rocketing Listeria. This finding suggests that the association of Annexin 2 with macropinocytic rockets requires native pinosomal membrane. Annexin 2 is necessary for the formation of macropinocytic rockets since overexpression of a dominant-negativeAnnexin 2 construct abolished the formation of these structures. The same construct did not prevent the movement of Listeria, in infected cells. These results show that recruitment of Annexin 2 to nascent macropinosome membranes is an essential prerequisite for actin polymerization-dependent vesicle locomotion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:35:42