Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Impaired hyperpolarization in regenerated endothelium after balloon catheter injury
Autore:
Kohler, R; Brakemeier, S; Kuhn, M; Behrens, C; Real, R; Degenhardt, C; Orzechowski, HD; Pries, AR; Paul, M; Hoyer, J;
Indirizzi:
Benjamin Franklin Med Ctr, Dept Nephrol, D-12200 Berlin, Germany Benjamin Franklin Med Ctr Berlin Germany D-12200 D-12200 Berlin, Germany Benjamin Franklin Med Ctr, Dept Clin Pharmacol, D-12200 Berlin, Germany Benjamin Franklin Med Ctr Berlin Germany D-12200 D-12200 Berlin, Germany Free Univ Berlin, Inst Physiol, Berlin, Germany Free Univ Berlin Berlin Germany v Berlin, Inst Physiol, Berlin, Germany
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 2, volume: 89, anno: 2001,
pagine: 174 - 179
SICI:
0009-7330(20010720)89:2<174:IHIREA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORCINE CORONARY-ARTERIES; ACTIVATED POTASSIUM CHANNEL; ION CHANNELS; CELLS; CALCIUM; CONDUCTANCE; EXPRESSION; RAT; K+;
Keywords:
angioplasty; neointima; endothelium; Ca2+-activated K+ channels; endothelium-dependent vasodilation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kohler, R Benjamin Franklin Med Ctr, Dept Nephrol, Hindenburgdamm 30, D-12200 Berlin, Germany Benjamin Franklin Med Ctr Hindenburgdamm 30 Berlin Germany D-12200
Citazione:
R. Kohler et al., "Impaired hyperpolarization in regenerated endothelium after balloon catheter injury", CIRCUL RES, 89(2), 2001, pp. 174-179

Abstract

Ca2+-activated K+ (K-Ca) channels control endothelial Ca2+ homeostasis andthe formation of vasodilators. After angioplasty, dysfunction of the regenerated endothelium, leads to abnormal vasoregulation. In this study, we tested the expression and function of K-Ca channels in regenerated endotheliumat 6 weeks after balloon catheter injury of rat carotid arteries (CAs) by using single-cell reverse transcription-polymerase chain reaction, patch-clamp techniques, and analysis of vasoreactivity. In single regenerated endothelial cells (ECs), the percentage of ECs expressing the K-Ca genes, rSK3 (12 +/-8%) and rIK1 (22 +/-9%), was significantly lower compared with the percentage of native ECs expressing these genes (rSK3 58 +/-8%, rIK1 64 +/- 10%). In patch-clamp experiments, K-Ca currents and acetylcholine-induced hyperpolarization were markedly reduced in regenerated ECs (shift of membranepotential -6 +/-3 mV) compared with those in native ECs (shift of membranepotential -21 +/-5 mV). In pressure myograph experiments, acetylcholine-induced dilation was impaired in reendothelialized CAs compared with normal CAs. Intraluminal application of the K-Ca blocker apamin and charybdotoxin inhibited dilation by 30% in normal CAs but was without effect in reendothelialized CAs. Intraluminal application of 1-ethyl-2-benzimidazolinone (100 mu mol/L), an opener of K-Ca channels, evoked dilation by 29% in normal CAs but had no effect in reendothelialized CAs. In conclusion, the impaired expression of K-Ca channels in regenerated endothelium results in defective hyperpolarization and impaired dilation. Thus, the impaired K-Ca channel function contributes to functional alterations of regenerated endothelium afterangioplasty.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:40:56