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Titolo:
Trace analysis of amikacin in human plasma by high-performance liquid chromatography
Autore:
Feng, CH; Lin, SJ; Wu, HL; Chen, SH;
Indirizzi:
Kaohsiung Med Univ, Sch Pharm, Kaohsiung 807, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan 807 Sch Pharm, Kaohsiung 807, Taiwan
Titolo Testata:
CHROMATOGRAPHIA
, volume: 53, anno: 2001,
parte:, 2 supplemento:, S
pagine: S213 - S217
SICI:
0009-5893(2001)53:<S213:TAOAIH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINOGLYCOSIDE ANTIBIOTICS; POSTCOLUMN DERIVATIZATION; PRECOLUMN DERIVATIZATION; BIOLOGICAL-FLUIDS; SERUM; IMMUNOASSAY; ASSAYS; TOBRAMYCIN; DRUGS;
Keywords:
column liquid chromatography; amikacin in plasma; naphthyl isothiocyanate derivatization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, SH Kaohsiung Med Univ, Sch Pharm, Kaohsiung 807, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan 807 , Kaohsiung 807, Taiwan
Citazione:
C.H. Feng et al., "Trace analysis of amikacin in human plasma by high-performance liquid chromatography", CHROMATOGR, 53, 2001, pp. S213-S217

Abstract

A simple and sensitive liquid chromatographic method is described for the determination of amikacin in human plasma. The amikacin is derivatized with1-naphthyl isothiocyanate (NITC) at 70 degreesC. After derivatization, a methylamine acetonitrile solution is added to the reaction mixture to eliminate excess derivatizing agent. The resulting derivative was analysed by HPLC on a LiChroCART RP-C-18 column with water-acetonitrile (57:43, v/v) mobile phase and detection at 230 nm. Parameters affecting derivatization of amikacin, including reaction temperature, reaction time and amount of derivatizing agent, were investigated. The linear range for the determination of amikacin in spiked plasma was over 10-52 nmol mL(-1); the detection limit (signal-to-noise ratio = 3; injection volume, 10 muL) was ca. 1 nmol mL-1. Therelative standard deviation was < 2.37% for intra-day assay (n = 6), 5.80%for inter-day assay (n = 6) and relative recovery was > 91 %.

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Documento generato il 03/04/20 alle ore 04:20:28