Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mass spectrometry for protein and peptide characterisation
Autore:
Jonsson, AP;
Indirizzi:
Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 phys, S-17177 Stockholm, Sweden
Titolo Testata:
CELLULAR AND MOLECULAR LIFE SCIENCES
fascicolo: 7, volume: 58, anno: 2001,
pagine: 868 - 884
SICI:
1420-682X(200106)58:7<868:MSFPAP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER-DESORPTION IONIZATION; ELECTRON-CAPTURE DISSOCIATION; COLLISION ACTIVATED DISSOCIATION; SURFACE-INDUCED DISSOCIATION; AMIDE HYDROGEN-EXCHANGE; ION-SOURCE; PROTONATED PEPTIDES; SEQUENCE DATABASES; MICRO-ELECTROSPRAY; 2-DIMENSIONAL GELS;
Keywords:
mass spectrometry; protein; peptide; matrix-assisted laser desorption/ionisation; electrospray ionisation; quadrupole; time-of-flight;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
159
Recensione:
Indirizzi per estratti:
Indirizzo: Jonsson, AP Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 7 Stockholm, Sweden
Citazione:
A.P. Jonsson, "Mass spectrometry for protein and peptide characterisation", CELL MOL L, 58(7), 2001, pp. 868-884

Abstract

Mass spectrometry has become an important analytical tool in biological and biochemical research. Its speed accuracy and sensitivity are unmatched byconventional analytical techniques. Identification of proteins and characterisation of their primary structure is a rapidly growing field in the post-genomic era, where matrix-assisted laser desorption/ionisation time-of-flight peptide mass fingerprinting combined with electrospray tandem mass spectrometry can efficiently solve many questions. Many recently determined genomic sequences have not been characterised at the protein level. Analysis of the amino acid sequence and characterisation of post-translational modifications are therefore important steps towards correlation of protein structure with function. This review concerns methods, instrumentation and applications of mass spectrometry in protein and peptide analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:41:52