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Titolo:
Immunohistochemical detection of ornithine-decarboxylase in primary and metastatic human breast cancer specimens
Autore:
Manni, A; Astrow, SH; Gammon, S; Thompson, J; Mauger, D; Washington, S;
Indirizzi:
Penn State Univ, Coll Med, Dept Med, Div Endocrinol, Hershey, PA 17033 USAPenn State Univ Hershey PA USA 17033 iv Endocrinol, Hershey, PA 17033 USA IMPATH, Los Angeles, CA USA IMPATH Los Angeles CA USAIMPATH, Los Angeles, CA USA
Titolo Testata:
BREAST CANCER RESEARCH AND TREATMENT
fascicolo: 2, volume: 67, anno: 2001,
pagine: 147 - 156
SICI:
0167-6806(200105)67:2<147:IDOOIP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYAMINE PATHWAY; EPITHELIAL-CELLS; INVOLVEMENT; GROWTH; CULTURE;
Keywords:
benign breast disease; breast cancer; breast cancer prognosis; normal breast tissue; ODC immunohistochemistry; polyamines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Manni, A Penn State Univ, Coll Med, Dept Med, Div Endocrinol, H044,500 Univ Dr,POB 850, Hershey, PA 17033 USA Penn State Univ H044,500 Univ Dr,POB 850 Hershey PA USA 17033 USA
Citazione:
A. Manni et al., "Immunohistochemical detection of ornithine-decarboxylase in primary and metastatic human breast cancer specimens", BREAST CANC, 67(2), 2001, pp. 147-156

Abstract

Increased ornithine decarboxylase (ODC) activity in human breast cancer specimens has recently been shown to be an independent adverse prognostic factor for recurrence and death. Biochemical measurement of ODC, however, is not practical for routine clinical use. Furthermore, it does not take into account the heterogeneous composition of human breast cancers which contain variable proportions of epithelial and stromal elements. Therefore, we developed an immunohistochemical method for ODC determination which can be applied to formalin-fixed, paraffin-embedded tissue sections. We report here our results in a series of 30 human breast cancer samples. ODC expression wasdetected most consistently in the malignant epithelial component of the tumors. Twenty-seven of 30 samples stained positive with intensities ranging from 1+ to 3+. The fraction of malignant epithelial cells expressing ODC varied among specimens between 10 % and > 90 %. When quantitated by H-SCORE, ODC expression was significantly higher in the malignant epithelial component than in normal appearing epithelial cells and stroma admixed within the tumor. Normal mammary tissue adjacent to the cancer was available for analysis in six cases. ODC expression was absent in two (while both cancers werepositive) but present in four to a degree which was overall comparable to that observed in the corresponding tumors. We believe that this technique will be useful for future studies aimed at expanding our knowledge of the role of ODC and polyamines (PA) in breast cancer biology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/08/20 alle ore 01:41:49