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Titolo:
Bisphosphonate acts on osteoclasts independent of ruffled borders in osteosclerotic (oc/oc) mice
Autore:
Ito, M; Amizuka, N; Nakajima, T; Ozawa, H;
Indirizzi:
Niigata Univ, Fac Dent, Dept Oral Biochem, Niigata 9518514, Japan Niigata Univ Niigata Japan 9518514 Oral Biochem, Niigata 9518514, Japan Niigata Univ, Fac Dent, Dept Oral Anat 1, Niigata, Japan Niigata Univ Niigata Japan , Fac Dent, Dept Oral Anat 1, Niigata, Japan Niigata Univ, Fac Dent, Dept Oral & Maxillofacial Surg 1, Niigata, Japan Niigata Univ Niigata Japan Oral & Maxillofacial Surg 1, Niigata, Japan Matsumoto Dent Univ, Inst Dent Sci, Nagano, Japan Matsumoto Dent Univ Nagano Japan ent Univ, Inst Dent Sci, Nagano, Japan
Titolo Testata:
BONE
fascicolo: 6, volume: 28, anno: 2001,
pagine: 609 - 616
SICI:
8756-3282(200106)28:6<609:BAOOIO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-RESORPTION; CELL-DEATH; H+-ATPASE; RAT BONE; LOCALIZATION; OSTEOPOROSIS; INHIBITION; APOPTOSIS; DISEASE; TUMOR;
Keywords:
osteoclasts; bisphosphonates; apoptosis; cell death; osteosclerotic (oc/oc) mice; ultrastructure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ito, M Niigata Univ, Fac Dent, Dept Oral Biochem, 5274 2 Bancho,Gakkocho Dori, Niigata 9518514, Japan Niigata Univ 5274 2 Bancho,Gakkocho Dori Niigata Japan 9518514 pan
Citazione:
M. Ito et al., "Bisphosphonate acts on osteoclasts independent of ruffled borders in osteosclerotic (oc/oc) mice", BONE, 28(6), 2001, pp. 609-616

Abstract

We examined the effects of a third generation bisphosphonate [YM-175; disodium dihydrogen (cycloheptylamino)-methylene-1,1-bisphosphonate] on osteoclasts in osteosclerotic (oc/oc) mice to elucidate the cellular mechanism forincorporation of the bisphosphonate, Osteoclasts of oc/oc mice were in direct contact with bone matrix but devoid of ruffled borders. Tartrate-resistant acid phosphatase (TRA-Pase) showed spotty localization intercellularly,whereas vacuolar H+-ATPase (V-ATPase) immunoreactivity was observed homogeneously in the cytoplasm, Upon injection of bisphosphonate, most osteoclasts lost cell polarity and were detached from bone surfaces. The detached osteoclasts underwent apoptosis as characterized by condensation of chromatin,absence of Golgi apparatus, and formation of many vesicles in the cytoplasm, Both TRAPase and V-ATPase were evenly distributed in the cytoplasm, The pyknotic nuclei of osteoclasts revealed DNA fragments as evidenced by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. The results indicate that osteoclasts lacking ruffled borders in oc/oc mice incorporated the bisphosphonate from a site different fromruffled borders and that bisphosphonate may directly affect osteoclasts without mediating its deposition to the bone matrix. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 23:41:51