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Titolo:
Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture
Autore:
Walker, LM; Preston, MR; Magnay, JL; Thomas, PBM; El Haj, AJ;
Indirizzi:
Keele Univ, N Staffordshire Hosp, Postgrad Med Sch, Ctr Sci & Technol Med,Stoke On Trent ST4 7QB, Staffs, England Keele Univ Stoke On Trent Staffs England ST4 7QB ST4 7QB, Staffs, England N Staffordshire Hosp Trust, Dept Orthopaed Surg, Stoke On Trent, Staffs, England N Staffordshire Hosp Trust Stoke On Trent Staffs England Staffs, England
Titolo Testata:
BONE
fascicolo: 6, volume: 28, anno: 2001,
pagine: 603 - 608
SICI:
8756-3282(200106)28:6<603:NROCAO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLCHOLINE-RECEPTOR GENES; STIMULATES DNA-SYNTHESIS; CIGARETTE-SMOKING; SKELETAL-MUSCLE; RISK-FACTORS; IN-VITRO; OSTEOPOROSIS; CHANNELS; DENSITY; WOMEN;
Keywords:
nicotine; osteoblasts; human; nicotinic receptor; c-fos; osteopontin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: El Haj, AJ Keele Univ, N Staffordshire Hosp, Postgrad Med Sch, Ctr Sci & Technol Med,Thornburrow Rd,Hartshill, Stoke On Trent ST4 7QB, Staffs, England Keele Univ Thornburrow Rd,Hartshill Stoke On Trent Staffs England ST4 7QB
Citazione:
L.M. Walker et al., "Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture", BONE, 28(6), 2001, pp. 603-608

Abstract

Long-term in vivo studies have highlighted smoking as a risk factor in postmenopausal osteoporosis, bone fracture incidence, and increased nonunion rates. In contrast, there are few data postulating the effects of smoking atthe cellular level in human skeletal tissue. In this study, we present novel evidence demonstrating that the nicotinic receptor alpha4 subunit is present in human primary bone cells by using reverse transcriptase-polymerase chain reaction (RT-PCR), In addition, we demonstrate direct cellular effects of nicotine on primary human bone cells and blockage of these effects with a nicotinic receptor antagonist, D-tubocurarine. Nicotine effects on cellproliferation were biphasic with toxic, antiproliferative effects at high levels of nicotine (>1 mmol/L) and stimulatory effects at very low levels (0.01-10 mu mol/L) after 72 h. This nicotine-induced increase in cell proliferation was inhibited in a dose-dependent manner by the addition of D-tubocurarine, In addition, proliferation effects from low-level treatment correlated with an upregulation of expression of the AP-1 transcription factor, c-fos, within 1 h, which was blocked by incubation with D-tubocurarine. To determine in situ bone cell responses within their trabecular matrix, cores of human bone isolated from biopsies were perfused with 0.1 mu mol/L nicotine for 24 h. Western analysis of proteins isolated from the cores highlighted an increase in osteopontin, a bone matrix protein implicated in regulating resorption, which was partially inhibited by the addition of D-tubocurarine, To conclude, our results suggest that nicotine has a direct effect on human bone cells in modulating proliferation, upregulation of the c-fos transcription factor, and the synthesis of the bone matrix protein, osteopontin, (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:40:59