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Titolo:
Endothelin- and sarafotoxin-induced receptor-mediated calcium mobilizationin a clonal murine osteoblast-like cell line, MC3T3-E1/B
Autore:
Zach, D; Windischhofer, W; Leis, HJ;
Indirizzi:
Graz Univ, Childrens Hosp, Dept Biochem Anal & Mass Spectrometry, A-8036 Graz, Austria Graz Univ Graz Austria A-8036 & Mass Spectrometry, A-8036 Graz, Austria
Titolo Testata:
BONE
fascicolo: 6, volume: 28, anno: 2001,
pagine: 595 - 602
SICI:
8756-3282(200106)28:6<595:EASRCM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; SIGNAL-TRANSDUCTION; OSTEOSARCOMA CELLS; PHOSPHOLIPASE-C; VASOCONSTRICTOR PEPTIDE; PARATHYROID-HORMONE; ETA RECEPTOR; B RECEPTORS; POTENT; EXPRESSION;
Keywords:
endothelins (ETs) sarafotoxins (S6s); receptor subtype; intracellular calcium release; transmembrane calcium inflow; osteoblast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Zach, D Graz Univ, Childrens Hosp, Dept Biochem Anal & Mass Spectrometry, Auenbruggerpl 30, A-8036 Graz, Austria Graz Univ Auenbruggerpl 30 Graz Austria A-8036 8036 Graz, Austria
Citazione:
D. Zach et al., "Endothelin- and sarafotoxin-induced receptor-mediated calcium mobilizationin a clonal murine osteoblast-like cell line, MC3T3-E1/B", BONE, 28(6), 2001, pp. 595-602

Abstract

Previous studies have demonstrated that, in osteoblast-like MC3T3-E1 cells, various endothelin peptides and their homologous sarafotoxins generate prostaglandin E-2 (PGE(2)) release through an ETA receptor subtype. In this study, biphasic Ca2+ signals elicited with endothelin (ET)-1, ET-2, ET-3, beta -ET, S6a1, and S6b (ET/S6) were measured by microspectrofluorimetric methods in cell suspensions loaded with Fura-2 acetoxymethylester (Fura-2 AM). Phospholipase C (PLC)-dependent calcium activation mechanisms seem to be involved. We found evidence of Ca2+ release from thapsigargin-sensitive and non-thapsigargin-sensitive intracellular Ca2+ stores as well as Ca2+ transmembrane inflow through multiple voltage-independent and Ni2+-sensitive cation channels, Using an ET, receptor antagonist, BQ-123, we showed that this receptor was coupled to Ca2+ mobilization, All agonists tested, except S6c tan ETB-receptor-specific agonist) induced receptor desensitization. Our results demonstrate that the ET/S6-induced Ca2+ signaling pathway is mediatedvia an ETA-receptor subtype in MC3T3-E1/B cells. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:39:10