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Titolo:
Transforming growth factor-beta 1 (TGF-beta) stimulates the osteoclast-forming potential of peripheral blood hematopoietic precursors in a lymphocyte-rich microenvironment
Autore:
Massey, HM; Scopes, J; Horton, MA; Flanagan, AM;
Indirizzi:
Imperial Coll, Sch Med, Dept Histopathol, London, England Imperial Coll London England Sch Med, Dept Histopathol, London, England Univ Coll, Dept Med, Bone & Mineral Ctr, London, England Univ Coll London England Dept Med, Bone & Mineral Ctr, London, England
Titolo Testata:
BONE
fascicolo: 6, volume: 28, anno: 2001,
pagine: 577 - 582
SICI:
8756-3282(200106)28:6<577:TGF1(S>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; CELLS; DIFFERENTIATION; MODULATION; ANTIGEN; OSTEOPROTEGERIN; SECRETION; RECEPTOR;
Keywords:
osteoclast; bone resorption; transforming growth factor beta; receptor activator of NF kappa B ligand;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Flanagan, AM Royal Free & Univ Coll Med Sch, Dept Histopathol, RockefellerBldg,Univ St, London WC1E 6JJ, England Royal Free & Univ Coll Med Sch Rockefeller Bldg,Univ St London England WC1E 6JJ
Citazione:
H.M. Massey et al., "Transforming growth factor-beta 1 (TGF-beta) stimulates the osteoclast-forming potential of peripheral blood hematopoietic precursors in a lymphocyte-rich microenvironment", BONE, 28(6), 2001, pp. 577-582

Abstract

Osteoclasts are generated from peripheral blood mononuclear cells (PBMNCs)in the presence of soluble receptor activator of NF kappaB ligand (sRANKL)and macrophage colony-stimulating factor (M-CSF), We show that human osteoclast formation is enhanced when PBMNCs are cultured in the presence of transforming growth factor (TGF)-beta and M-CSF prior to the addition of sRANKL, The effect was only observed in the presence of a nonadherent lymphocytePBMNC fraction. Osteoclast formation was enhanced to a level equivalent tothat induced by TGF-beta when nonadherent PBMNC fraction was removed from the cultures, prior to RANKL treatment. These data suggest that TGF-beta enhances osteoclast formation by abrogating the suppressive effect of the nonadherent PBMNCs, thereby maintaining the osteoclast-forming potential of the osteoclast precursor population. TGF-beta was without effect on proliferation of the adherent PBMNCs and did not stimulate osteoclast size or modifytheir immunophenotype, The effect was not mediated through prostaglandin synthesis. These results indicate that the microenvironment encountered by the osteoclast precursor prior to RANKL exposure contributes significantly to the regulation of osteoclast formation. Furthermore, the data emphasize that the effect of TGF-beta is determined by the cytokine milieu of the microenvironment and/or the state of activation of the cell being targeted by TGF-beta; thus, the effect of TGF-beta is context-dependent. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:42:19