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Titolo:
DUB-2A, a new member of the DUB subfamily of hematopoietic deubiquitinating enzymes
Autore:
Baek, KH; Mondoux, MA; Jaster, R; Fire-Levin, E; DAndrea, AD;
Indirizzi:
Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Dept Pediat Oncol, Boston, MA 02115 USA
Titolo Testata:
BLOOD
fascicolo: 3, volume: 98, anno: 2001,
pagine: 636 - 642
SICI:
0006-4971(20010801)98:3<636:DANMOT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
UBIQUITIN-PROTEASOME PATHWAY; NUCLEAR-PORE COMPLEX; SACCHAROMYCES-CEREVISIAE; FUNCTIONAL-ANALYSIS; CELL-CYCLE; C-CBL; PROTEIN; GENE; YEAST; DEGRADATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: D'Andrea, AD Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pediat Oncol, 44 BinneySt, Boston, MA 02115 USA Harvard Univ 44 Binney St Boston MA USA 02115 n, MA 02115 USA
Citazione:
K.H. Baek et al., "DUB-2A, a new member of the DUB subfamily of hematopoietic deubiquitinating enzymes", BLOOD, 98(3), 2001, pp. 636-642

Abstract

Protein ubiquitination Is an important regulator of cytokine-activated signal transduction pathways and hematopoietic cell growth. Protein ubiquitination is controlled by the coordinate action of ubiquitin-conjugating enzymes and deubiquitinating enzymes. Recently a novel family of genes encoding growth-regulatory deubiquitinating enzymes (DUB-1 and DUB-2) has been identified. DUBs are immediate-early genes and are induced rapidly and transiently in response to cytokine stimuli. By means of polymerase chain reaction amplification with degenerate primers for the DUB-2 complementary DNA, 3 murine bacterial artificial chromosome (BAC) clones that contain DUB gene sequences were isolated. One BAC contained a novel DUB gene (DUB-2A) with extensive homology to DUB-2. Like DUB-1 and DUB-2, the DUB-2A gene consists of 2 exons. The predicted DUB-2A protein is highly related to other DUBs throughout the primary amino acid sequence, with a hypervariable region at its C-terminus. In vitro, DUB-2A had functional deubiquitinating activity; mutation of its conserved amino acid residues abolished this activity. The 5 ' flanking sequence of the DUB-2A gene has a hematopoietic-specific functional enhancer sequence. It Is proposed that there are at least 3 members of the DUB subfamily (DUB-1, DUB-2, and DUB-2A) and that different hematopoietic cytokines induce specific DUB genes, thereby initiating a cytokine-specific growth response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:02:34