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Titolo:
Transfer of the human telomerase reverse transcriptase (TERT) gene into T lymphocytes results in extension of replicative potential
Autore:
Rufer, N; Migliaccio, M; Antonchuk, J; Humphries, RK; Roosnek, E; Lansdorp, PM;
Indirizzi:
British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy Vancouver BC Canada V5Z 1L3 BC V5Z 1L3, Canada Univ Geneva, Div Immunol & Allergol, Geneva, Switzerland Univ Geneva Geneva Switzerland Immunol & Allergol, Geneva, Switzerland CHU Vaudois, Ludwig Inst Canc Res, Div Clin Oncoimmunol, CH-1011 Lausanne,Switzerland CHU Vaudois Lausanne Switzerland CH-1011 l, CH-1011 Lausanne,Switzerland Univ British Columbia, Dept Med, Vancouver, BC, Canada Univ British Columbia Vancouver BC Canada ept Med, Vancouver, BC, Canada Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland Swiss Inst Expt Canc Res Epalinges Switzerland CH-1066 nges, Switzerland
Titolo Testata:
BLOOD
fascicolo: 3, volume: 98, anno: 2001,
pagine: 597 - 603
SICI:
0006-4971(20010801)98:3<597:TOTHTR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL HUMAN-CELLS; HUMAN FIBROBLASTS; LIFE-SPAN; REGULATED EXPRESSION; SOMATIC-CELLS; LENGTH; IMMORTALIZATION; MEMORY; SENESCENCE; MAINTENANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Rufer, N Hop Beaumont, Lab AIDS Immunopathol, Ave Beaumont 29, CH-1011 Lausanne, Switzerland Hop Beaumont Ave Beaumont 29 Lausanne Switzerland CH-1011 erland
Citazione:
N. Rufer et al., "Transfer of the human telomerase reverse transcriptase (TERT) gene into T lymphocytes results in extension of replicative potential", BLOOD, 98(3), 2001, pp. 597-603

Abstract

In most human somatic cells telomeres progressively shorten with each celldivision eventually leading to chromosomal instability and cell senescence. The loss of telomere repeats with cell divisions may also limit the replicative life span of antigen-specific T lymphocytes. Recent studies have shown that the replicative life span of various primary human cells can be prolonged by induced expression of the telomerase reverse transcriptase (hTERT) gene. To test whether introduction of hTERT can extend the life span of primary human T lymphocytes, naive CD8(+) T lymphocytes were transfected with retroviral vectors containing the hTERT gene. Transduced T-cell clones expressed high levels of telomerase and either maintained or elongated their telomere lengths upon culture for extended periods of time. Two of the transduced subclones retained a normal cloning efficiency for more than 170 population doublings (PDs). In contrast, T-cell clones transfected with control vectors exhibited progressive telomere length shortening and stopped proliferation at around 108 PDs. Telomerase-positive T clones had a normal 46,XY karyotype, maintained their cytotoxic properties, and showed very little staining for the apoptotic marker annexin-V. These results indicate that ectopic hTERT gene expression is capable of extending the replicative life span of primary human CD8(+) cytotoxic T lymphocytes.

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Documento generato il 02/04/20 alle ore 09:38:53