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Titolo:
Cloning and analysis of the rat glutamate-cysteine ligase modifier subunitpromoter
Autore:
Yang, HP; Wang, JH; Ou, XP; Huang, ZZ; Lu, SC;
Indirizzi:
Univ So Calif, Sch Med, Dept Med, Div Gastrointestinal & Liver Dis, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 ver Dis, Los Angeles, CA 90033 USA Univ So Calif, Keck Sch Med, Res Ctr Alcohol Liver & Pancreat Dis, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 eat Dis, Los Angeles, CA 90033 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 285, anno: 2001,
pagine: 476 - 482
SICI:
0006-291X(20010713)285:2<476:CAAOTR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; ELECTROPHILE RESPONSIVE ELEMENT; AMINO-ACID-SEQUENCE; GENE-EXPRESSION; LIGHT SUBUNIT; GLUTATHIONE SYNTHESIS; HEAVY SUBUNIT; CELL-DENSITY; HEPG2 CELLS; BINDING;
Keywords:
glutathione; H4IIE cells; glutamate-cysteine ligase; DNase I footprinting; transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, SC Univ So Calif, Sch Med, Dept Med, Div Gastrointestinal & Liver Dis,2011 Zonal Ave,HMR Bldg,415, Los Angeles, CA 90033 USA Univ So Calif 2011 Zonal Ave,HMR Bldg,415 Los Angeles CA USA 90033
Citazione:
H.P. Yang et al., "Cloning and analysis of the rat glutamate-cysteine ligase modifier subunitpromoter", BIOC BIOP R, 285(2), 2001, pp. 476-482

Abstract

Glutamate-cysteine ligase (GCL), the rate-limiting enzyme in glutathione (GSH) synthesis, is made up of two subunits, a catalytic (GCLC) and a modifier (GCLM) subunit, which are differentially regulated. Increased GCLM expression occurs under certain oxidative stress conditions. To facilitate studies of GCLM transcriptional regulation, we have cloned and characterized a 1.86-kb 5 ' -flanking region of the rat GCLM (GenBank Accession No. AF311745). A TATA-like element and one transcriptional start sites are located at 364 and 93 nucleotides upstream of the translational start site, respectively. The promoter contains consensus binding sites for many transcription factors including activator protein 1 (AP-1), transcription factor 11 (TCF11),heat shock transcription factor (HSF), and nuclear factor kappa B (NF kappaB). The rat GCLM promoter was able to drive efficiently luciferase expression in H4IIE cells. Sequential deletion analysis revealed DNA regions, -649to -154 and -1251 to -649, are involved in positive and negative gene regulation, respectively. Candidate transcription factors were identified by DNase I footprinting. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:34:15