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Titolo:
Toxicity of beta-carotene and its exacerbation by acetaldehyde in HepG2 cells
Autore:
Ni, RY; Leo, MA; Zhao, JB; Lieber, CS;
Indirizzi:
Vet Adm Med Ctr, Liver Dis & Nutr Sect, Bronx, NY 10468 USA Vet Adm Med Ctr Bronx NY USA 10468 r Dis & Nutr Sect, Bronx, NY 10468 USA Mt Sinai Sch Med, Bronx, NY 10468 USA Mt Sinai Sch Med Bronx NY USA 10468Mt Sinai Sch Med, Bronx, NY 10468 USA
Titolo Testata:
ALCOHOL AND ALCOHOLISM
fascicolo: 4, volume: 36, anno: 2001,
pagine: 281 - 285
SICI:
0735-0414(200107/08)36:4<281:TOBAIE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-A DEPLETION; ETHANOL-CONSUMPTION; ALPHA-TOCOPHEROL; LUNG-CANCER; HEPATOTOXICITY; ALCOHOL; DAMAGE; RATS; ANTIOXIDANT; RETINOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Lieber, CS Vet Adm Med Ctr, Liver Dis & Nutr Sect, 151-2,130 W KingsbridgeRd, Bronx,NY 10468 USA Vet Adm Med Ctr 151-2,130 W Kingsbridge Rd Bronx NYUSA 10468 A
Citazione:
R.Y. Ni et al., "Toxicity of beta-carotene and its exacerbation by acetaldehyde in HepG2 cells", ALC ALCOHOL, 36(4), 2001, pp. 281-285

Abstract

In rats and baboons, the hepatotoxicity of chronic ethanol consumption is exacerbated by beta -carotene feeding, but the mechanism of this adverse effect is unknown. In this study, the toxicity of beta -carotene and acetaldehyde was documented by the MTT test (an assay of reduction of tetrazolium to formazan) and by lactate dehydrogenase (LDH) leakage. In HepG2 cells, beta -carotene or acetaldehyde inhibited mitochondrial reduction function as indicated by a decrease of the MTT test. beta -Carotene was inhibitory at very low concentration, in a dose-dependent manner. The combination of these two compounds resulted in an additive effect. Acetaldehyde increased LDH leakage from the HepG2 cells into the medium, whereas beta -carotene by itself did not show such an effect, but it exacerbated the toxicity of acetaldehyde when combined. In addition, this study showed that acetaldehyde and beta -carotene inhibited each other's clearance from the medium, which suggests that these two chemicals may share, at least in part, a common metabolic pathway (possibly via aldehyde dehydrogenase) in the cells, and that a competitive inhibition may exist. In conclusion, this preliminary study indicates that beta -carotene is toxic to hepatocytes, especially when combined with acetaldehyde, the metabolite of ethanol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:29:22