Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity
Autore:
Tanaka, N; Konomi, M; Osumi, M; Takegawa, K;
Indirizzi:
Kagawa Univ, Fac Agr, Dept Life Sci, Miki, Kagawa 7610795, Japan Kagawa Univ Miki Kagawa Japan 7610795 fe Sci, Miki, Kagawa 7610795, Japan Japan Womens Univ, Fac Sci, Dept Chem & Biol Sci, Bunkyo Ku, Tokyo 1128681, Japan Japan Womens Univ Tokyo Japan 1128681 i, Bunkyo Ku, Tokyo 1128681, Japan
Titolo Testata:
YEAST
fascicolo: 10, volume: 18, anno: 2001,
pagine: 903 - 914
SICI:
0749-503X(200107)18:10<903:COSPMD>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FISSION YEAST; SACCHAROMYCES-CEREVISIAE; PROTEIN GLYCOSYLATION; MANNOSYLPHOSPHATE TRANSFER; RESISTANT MUTANTS; ALPHA-AGGLUTININ; ACID-PHOSPHATASE; GENETIC-ANALYSIS; A-AGGLUTININ; LOCALIZATION;
Keywords:
Schizosaccharomyces pombe; galactosylation; UDP-galactose transporter; glycoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Takegawa, K Kagawa Univ, Fac Agr, Dept Life Sci, Miki, Kagawa 7610795, Japan Kagawa Univ Miki Kagawa Japan 7610795 , Kagawa 7610795, Japan
Citazione:
N. Tanaka et al., "Characterization of Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity", YEAST, 18(10), 2001, pp. 903-914

Abstract

In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell mall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1(+), a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in St. pombe. The Delta gms1 strain is therefore useful for the analysis of physiological properties of galactose residues in St. pombe, The deletion strain of gms1(+) was viable; however, it shelved an aberrant cell morphology and increased sensitivities to digestion with beta -glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white, A reduction of galactomannan layers of the cell wall in the Delta gms1 strain was observed byscanning and transmission electron microscopic analyses, The addition of osmotic stabilizer suppressed the morphologic defect of the Delta gms1 cells, while other phenotypes were weakly suppressed. The Delta gms1 (h(90)) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 14:45:50