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Titolo:
Inhibitory effects of MoAbs against a surface protein antigen in real-timeadherence in vitro and recolonization in vivo of Streptococcus mutans
Autore:
Senpuku, H; Matin, K; Abdus, SM; Kurauchi, I; Sakurai, S; Kawashima, M; Murata, T; Miyazaki, H; Hanada, N;
Indirizzi:
Natl Inst Infect Dis, Dept Oral Sci, Shinjuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Niigata Univ, Sch Dent, Dept Prevent Dent, Niigata 9518514, Japan Niigata Univ Niigata Japan 9518514 Prevent Dent, Niigata 9518514, Japan
Titolo Testata:
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
fascicolo: 1-2, volume: 54, anno: 2001,
pagine: 109 - 116
SICI:
0300-9475(200107/08)54:1-2<109:IEOMAA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCAL PASSIVE-IMMUNIZATION; CROSS-REACTING ANTIBODIES; HUMAN SALIVARY COMPONENTS; MONOCLONAL-ANTIBODIES; DENTAL-CARIES; SEROTYPE-C; PURIFIED PROTEIN; RHESUS-MONKEYS; CELL EPITOPES; T-CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Senpuku, H Natl Inst Infect Dis, Dept Oral Sci, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628640, Japan Natl Inst Infect Dis 1-23-1 Toyama Tokyo Japan 1628640 , Japan
Citazione:
H. Senpuku et al., "Inhibitory effects of MoAbs against a surface protein antigen in real-timeadherence in vitro and recolonization in vivo of Streptococcus mutans", SC J IMMUN, 54(1-2), 2001, pp. 109-116

Abstract

A surface protein antigen (PAc) of Streptococcus mutans, particularly the A-region of the molecule, has been reported to interact with salivary components on the tooth surface. It might be a candidate antigen inducing the production of antibodies against the adherence of S. mutans to the tooth surface. We investigated the effects of monoclonal antibodies (MoAbs) obtained by immunization of synthetic PAc peptides that completely correspond to theamino acid sequence of part of the A-region. These MoAbs recognize severalcore B-cell epitopes in the sequence. Two (KH5 and SH2) of these antibodies reacted with both S. mutans and Streptococcus sobrinus, but not with Streptococcus sanguis, Streptococcus salivarius, Porphyromonas gingivalis or Lactobacillus casei. They clearly inhibited the real-time adherence of S. mutans to salivary components in a biosensor. KH5, which showed a real-time inhibition (71%), also significantly prevented the recolonization of S. mutans on the tooth surface in rats. These results suggested that the core B-cell epitope (-Y---L--Y---) recognized by KH5 was the essential sequence in the antigenic epitopes of PAc protein recognized specifically by the inhibitory antibody. Therefore, the amino acid residues were found to be important in the initial attachment of S. mutans to the tooth surface. These results provide for the mechanism of PAc molecule in the initial attachment of S. mutans on the tooth surface and more effective designs for the removal of S.mutans and S. sobrinus from the oral cavity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:57:15