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Titolo:
Analysis of antibody to milk proteins in HIV positive/negative sera using two-dimensional electrophoresis, Western blot and immunoassay
Autore:
Goldfarb, M;
Indirizzi:
Anatek EP, Portland, ME 04103 USA Anatek EP Portland ME USA 04103Anatek EP, Portland, ME 04103 USA
Titolo Testata:
PROTEOMICS
fascicolo: 5, volume: 1, anno: 2001,
pagine: 721 - 725
SICI:
1615-9853(200105)1:5<721:AOATMP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAT-GLOBULE-MEMBRANE; 2-DIMENSIONAL ELECTROPHORESIS; BOVINE BUTYROPHILIN; MAMMARY TISSUE; COWS MILK; GLYCOPROTEIN; IDENTIFICATION; PEPTIDE; ADULTS;
Keywords:
HIV-1; milk; two-dimensional electrophoresis; immunoblot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Goldfarb, M Anatek EP, 17 Bishop St, Portland, ME 04103 USA Anatek EP 17 Bishop St Portland ME USA 04103 and, ME 04103 USA
Citazione:
M. Goldfarb, "Analysis of antibody to milk proteins in HIV positive/negative sera using two-dimensional electrophoresis, Western blot and immunoassay", PROTEOMICS, 1(5), 2001, pp. 721-725

Abstract

Homology between the 120 envelope glycoprotein of HIV-I virus and alpha-lactalbumin (a milk protein) stimulated interest in analysis of antibody in HIV-I positive plasma to cow's milk. Western blots of a two-dimensional (2-D) separation of milk were assayed with nine HIV-1 positive plasmas and ninecontrol sera. The control sera from reference labs represented various disease problems. All nine HIV-I positive plasma had antibody to between two and five milk proteins. All nine HIV-I positive plasma had antibody to albumin and immunoglobulin (IgG); in addition, seven reacted with butyrophilin, five with alpha-lactalbumin, six with casein. Four of the control sera had no antibody to milk proteins; three had minor reactivity to casein and two,one diagnosed as an IgM Lyme positive, one diagnosed with Helicobacter pylori detected three and four milk proteins. None of the control sera had antibody to albumin or alpha-lactalbumin. Bovine and human albumin have almostidentical sequence, and antibody generated to bovine serum albumin could react with human serum albumin. To look at this, three of the HIV-I positiveplasmas were assayed with 2-D Western blots of human milk and did have reactivity with albumin and IgG. Also, the protein butyrophilin is highly immunogenic and has significant homology with human neural and cellular proteins. Whether these antibodies can be causing allergy or autoimmune disease isspeculative, but they indicate an immune system which is highly activated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 15:21:23