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Titolo:
A reference map of human lung MRC-5 fibroblast proteins using immobilized pH gradient-isoelectric focusing-based two-dimensional electrophoresis
Autore:
Leung, KY; Wait, R; Welson, SY; Yan, YX; Abraham, DJ; Black, CM; Pearson, JD; Dunn, MJ;
Indirizzi:
Harefield Hosp, Imperial Coll, Sch Med, Natl Heart & Lung Inst Heart Sci Ctr, Harefield UB9 6JH, Middx, England Harefield Hosp Harefield Middx England UB9 6JH ld UB9 6JH, Middx, England Imperial Coll, Sch Med, Kennedy Inst, Rheumatol Div, London, England Imperial Coll London England nnedy Inst, Rheumatol Div, London, England Royal Free & Univ Coll London, Sch Med, Ctr Rheumatol, London, England Royal Free & Univ Coll London London England Rheumatol, London, England Univ London Kings Coll, Ctr Cardiovasc Biol & Med, London, England Univ London Kings Coll London England ovasc Biol & Med, London, England
Titolo Testata:
PROTEOMICS
fascicolo: 6, volume: 1, anno: 2001,
pagine: 787 - 794
SICI:
1615-9853(200106)1:6<787:ARMOHL>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED-LASER-DESORPTION/IONIZATION; MASS-SPECTROMETRY; POLYACRYLAMIDE GELS; 2-DIMENSIONAL ELECTROPHORESIS; IDENTIFICATION; PROLIFERATION; CELLS;
Keywords:
proteomics; two-dimensional gel electrophoresis; human lung MRC-5 fibroblasts; protein map; PDQUEST; matrix-assisted laser desorption; ionization time of flight;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Leung, KY Harefield Hosp, Imperial Coll, Sch Med, Natl Heart & Lung Inst Heart Sci Ctr, Harefield UB9 6JH, Middx, England Harefield Hosp Harefield Middx England UB9 6JH , Middx, England
Citazione:
K.Y. Leung et al., "A reference map of human lung MRC-5 fibroblast proteins using immobilized pH gradient-isoelectric focusing-based two-dimensional electrophoresis", PROTEOMICS, 1(6), 2001, pp. 787-794

Abstract

We report the first protein map of human adult lung MRC-5 fibroblasts using isoelectric focusing-immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis. MRC-5 is an immortalised cell line used in a wide range of investigations. The two-dimensional gel pattern of proteins generated from any given cell system provides a fingerprint that is unique to those cells. Therefore, the establishment of a protein map for a particular cell system provides a useful reference tool as a "master map" for subsequent studies using those cells. In this map a total of 98 protein spots were identified by comparative searches of the nucleotide and protein database using peptide masses obtained by matrix-assisted laser desorption/ionization time of flight following trypsin digestion. To increase the utility of the reference map, cells were cultured in both Dulbecco's modified Eagle medium (DMEM), the standard medium, and Roswell Park Memorial Institute (RPMI)-1640. Two-dimensional gel protein patterns of MRC-5 cultures were shown to be largely unaffected by the use of RPMI compared to DMEM, respectively. In combination with the reference map, the standardised protocol described provides a tool for comparative studies involving MRC-5 cells in which nonspecific variation is minimized.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:22:41