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Titolo:
Phosphotyrosine profiling to identify novel components of interferon and interleukin 6-family cytokine signalling
Autore:
Isharc, H; Watling, D; Kerr, IM;
Indirizzi:
ICRF, BRM 509, London WC2A 3PX, England ICRF London England WC2A 3PXICRF, BRM 509, London WC2A 3PX, England
Titolo Testata:
PROTEOMICS
fascicolo: 6, volume: 1, anno: 2001,
pagine: 767 - 772
SICI:
1615-9853(200106)1:6<767:PPTINC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTORS; PATHWAYS;
Keywords:
tyrosine phosphorylation; phosphotyrosine; profiling; signalling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Is'harc, H ICRF, BRM 509, 44 Lincolns Inn Fields, London WC2A 3PX, EnglandICRF 44 Lincolns Inn Fields London England WC2A 3PX X, England
Citazione:
H. Is'harc et al., "Phosphotyrosine profiling to identify novel components of interferon and interleukin 6-family cytokine signalling", PROTEOMICS, 1(6), 2001, pp. 767-772

Abstract

The interferons and interleukin-6 (IL-6) family cytokines exert their biological effects via Janus kinase and signal transducer and activator of transcription (JAK-STAT) signalling pathways. Our aim is to identify "novel" signalling molecules not previously implicated in JAK-mediated signalling. Phosphotyrosine profiles of either whole cell lysates, or subcellular fractions, of unstimulated and cytokine-treated cell lines have been analysed and ligand-inducible differences observed. Recombinant src homology 2 domains, biotinylated peptides corresponding to cytokine receptor intracellular domains, antiphosphotyrosine antibodies, anion exchange columns and 2-D phosphotyrosine profiling have been used to select cohorts of molecules that are tyrosine phosphorylated in response to cytokine treatment. Tyrosine phosphorylated proteins showing cytokine specificity or differential profiles in cell lines mutated in specific JAKs are being purified and identified by massspectrometry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 09:41:00