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Titolo:
Proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer
Autore:
Dwek, MV; Ross, HA; Leathem, AJC;
Indirizzi:
Royal Free & Univ Coll London, Med Sch, Inst Surg Studies, Dept Surg, London W1W 1EJ, England Royal Free & Univ Coll London London England W1W 1EJ on W1W 1EJ, England
Titolo Testata:
PROTEOMICS
fascicolo: 6, volume: 1, anno: 2001,
pagine: 756 - 762
SICI:
1615-9853(200106)1:6<756:PAGMIP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELIX-POMATIA AGGLUTININ; BETA-1-6 BRANCHED OLIGOSACCHARIDES; TN ANTIGEN EXPRESSION; EPITHELIAL-CELL LINES; LECTIN-BINDING; ADJUVANT CHEMOTHERAPY; TUMOR PROGRESSION; COLORECTAL-CANCER; COLON NEOPLASIA; SIALOSYL-TN;
Keywords:
Helix pomatia agglutinin; cancer; proteomic; metastasis; glycosylation; tumour markers; review;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Dwek, MV Royal Free & Univ Coll London, Med Sch, Inst Surg Studies, Dept Surg, Charles Bell House,67-73 Riding House St, London W1W 1EJ, England Royal Free & Univ Coll London Charles Bell House,67-73 Riding House St London England W1W 1EJ
Citazione:
M.V. Dwek et al., "Proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer", PROTEOMICS, 1(6), 2001, pp. 756-762

Abstract

Changes in glycosylation of glycoproteins and glycolipids is a common feature of cancer and may influence cancer cell behaviour, perhaps by enabling cell-cell interactions which favour metastasis or by allowing cancer cells to evade immuno-surveillance, Studies to identify glycosylation changes in human cancer have often used immunohistochemical techniques with lectins orantibodies and human tissue sections. Whilst some detailed biochemical studies have been performed there are few clinically relevant studies since the numbers of specimens evaluated are often very small. Using an immuno-histochemical approach, we have found that the lectin HPA from the albumen gland of Helix pomatia detects aggressive metastatic breast cancers. We have sought to identify the breast cancer associated oligosaccharides and the proteins to which they are attached via 2-DE for proteome analysis and HPLC separations for glycosylation mapping. Sixty-nine breast cancer specimens werestudied. The HPA staining pattern, clinical treatment and follow-up data were known. Oligosaccharides that related to HPA staining were identified and found in elevated levels in metastatic breast cancer specimens. Human breast and colorectal cancer cells grown in vitro and in a clinically relevantanimal model of metastasis also show increased levels of the oligosaccharides. We are now structurally characterising the oligosaccharides and aim touse them as diagnostic tools and targets for immune-therapy of breast and other solid tumours.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:02:38