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Titolo:
Determination of glycosyl-phosphatidylinositol membrane protein anchorage
Autore:
Hooper, NM;
Indirizzi:
Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
PROTEOMICS
fascicolo: 6, volume: 1, anno: 2001,
pagine: 748 - 755
SICI:
1615-9853(200106)1:6<748:DOGMPA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
KIDNEY MICROVILLAR MEMBRANE; CROSS-REACTING DETERMINANT; ACID-REQUIREMENTS ADJACENT; PHOSPHOLIPASE-C; PARASITIC PROTOZOA; ECTO-ENZYMES; LIPID RAFTS; DIFFERENTIAL SOLUBILIZATION; TRYPANOSOMA-BRUCEI; RENAL DIPEPTIDASE;
Keywords:
glycosyl-phosphatidylinositol; phospholipase; cross-reacting determinant; lipid rafts; prion protein; review;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Hooper, NM Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire,England Univ Leeds Leeds W Yorkshire England LS2 9JT orkshire, England
Citazione:
N.M. Hooper, "Determination of glycosyl-phosphatidylinositol membrane protein anchorage", PROTEOMICS, 1(6), 2001, pp. 748-755

Abstract

A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence of a GPI anchor on the mature protein requires experimental evidence, including sensitivity of the protein to release from cells ormembranes with bacterial phosphatidylinositol-specific phospholipase C, recognition by anti-cross-reacting determinant antibodies, or metabolic labelling with components of the anchor. GPI-anchored proteins are resistant to solubilisation with detergents such as Triton X-100 due to their association with cholesterol and glycosphingolipids in membrane domains known as lipid rafts. This detergent insolubility can be used to provide evidence for the presence of a GPI anchor on a protein and to isolate lipid rafts.

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Documento generato il 28/11/20 alle ore 01:03:06