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Titolo:
Anomalous mole-fraction effects in recombinant and native cyclic nucleotide-gated channels in rat olfactory receptor neurons
Autore:
Qu, W; Moorhouse, AJ; Cunningham, AM; Barry, PH;
Indirizzi:
Univ New S Wales, Sch Physiol & Pharmacol, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 l, Sydney, NSW 2052, Australia Univ New S Wales, Sch Paediat, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 t, Sydney, NSW 2052, Australia Garvan Inst Med Res, Div Neurobiol, Sydney, NSW 2010, Australia Garvan Inst Med Res Sydney NSW Australia 2010 Sydney, NSW 2010, Australia
Titolo Testata:
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
fascicolo: 1474, volume: 268, anno: 2001,
pagine: 1395 - 1403
SICI:
0962-8452(20010707)268:1474<1395:AMEIRA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED CHANNEL; SELECTIVITY; RODS; SALAMANDER; PERMEATION; CELLS;
Keywords:
anomalous mole-fraction effects; cyclic nucleotide-gated channels; patch clamp; cyclic AMP; olfactory receptor neurons; rOCNCl;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Barry, PH Univ New S Wales, Sch Physiol & Pharmacol, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 NSW 2052, Australia
Citazione:
W. Qu et al., "Anomalous mole-fraction effects in recombinant and native cyclic nucleotide-gated channels in rat olfactory receptor neurons", P ROY SOC B, 268(1474), 2001, pp. 1395-1403

Abstract

Anomalous mole-fraction effects (AMFE) were studied, using the inside-out configuration of the patch-clamp technique in both recombinant wild-type alpha -homomeric rat olfactory adenosine 3',5'-cyclic monophosphate (cAMP)-gated channels (rOCNCl) expressed in human embryonic kidney cells (HEK 293) and native cyclic nucleotide-gated (CNG) channels in acutely isolated rat olfactory receptor neurons. Single-channel and macroscopic currents were activated by 200 muM and 500 muM cAMP, respecitively. Macroscopic currents, measured with mixtures of Na+-NH4+ or Cs+-Li+ in the cytoplasmic bathing solution, displayed AMFE in the rOCNCl channels at both positive and negative membrane potentials. The rOCNCl single-channel conductance showed a distinct minimum (or maximum) in an 80% Na+-20% NH4+ mixture (or a 60% Cs+-40% Li+ mixture), but only at positive membrane potentials. Macroscopic measurementsin native olfactory CNG channels with mixtures of Na+-NH4+ indicated similar AMFE. These results suggest that both native CNG channels and recombinant alpha -homomeric channels allows several ions to be present simultaneously within the channel pore. The also further validate the dominant role of the alpha -subunit in permeation through these channels, provide the first evidence to suggest that rOCNCl channels have multi-ion properties and further justify the use of the rOCNCl channel as an effective model for structure-function studies of ion permeation and selectivity in olfactory CNG channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 19:05:17