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Titolo:
Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase
Autore:
Mahmoud, SS; Croteau, RB;
Indirizzi:
Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 ol Chem, Pullman, WA 99164 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 15, volume: 98, anno: 2001,
pagine: 8915 - 8920
SICI:
0027-8424(20010717)98:15<8915:MEOEOY>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEVALONATE-INDEPENDENT PATHWAY; MENTHA-PIPERITA L; ISOPRENOID BIOSYNTHESIS; GLANDULAR TRICHOMES; ESCHERICHIA-COLI; MONOTERPENE BIOSYNTHESIS; NONMEVALONATE PATHWAY; 4-(CYTIDINE 5'-DIPHOSPHO)-2-C-METHYL-D-ERYTHRITOL; 1-DEOXY-D-XYLULOSE-5-PHOSPHATE REDUCTOISOMERASE; AGROBACTERIUM-TUMEFACIENS;
Keywords:
peppermint; Mentha x piperita; monoterpene biosynthesis; mevalonate-independent pathway; isoprenoids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Croteau, RB Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 lman, WA 99164 USA
Citazione:
S.S. Mahmoud e R.B. Croteau, "Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase", P NAS US, 98(15), 2001, pp. 8915-8920

Abstract

Peppermint(Mentha x piperita L..) was independently transformed with a homologous sense version of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase cDNA and with a homologous antisense version of the menthofuran synthase cDNA, both driven by the CaMV 35S promoter. Two groups of transgenic plantswere regenerated in the reductoisomerase experiments, one of which remained normal in appearance and development; another was deficient in chlorophyll production and grew slowly. Transgenic plants of normal appearance and growth habit expressed the reductoisomerase transgene strongly and constitutively, as determined by RNA blot analysis and direct enzyme assay, and theseplants accumulated substantially more essential oil (about 50% yield increase) without change in monoterpene composition compared with wild-type. Chlorophyll-deficient plants did not afford detectable reductoisomerase mRNA or enzyme activity and yielded less essential oil than did wild-type plants,indicating cosuppression of the reductoisomerase gene. Plants transformed with the antisense version of the menthofuran synthase cDNA were normal in appearance but produced less than half of this undesirable monoterpene oil component than did wild-type mint grown under unstressed or stressed conditions. These experiments demonstrate that essential oil quantity and qualitycan be regulated by metabolic engineering. Thus, alteration of the committed step of the mevalonate-independent pathway for supply of terpenoid precursors improves flux through the pathway that leads to increased monoterpeneproduction, and antisense manipulation of a selected downstream monoterpene biosynthetic step leads to improved oil composition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 15:58:22