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Titolo:
Cloning and functional analysis of two gibberellin 3 beta-hydroxylase genes that are differently expressed during the growth of rice
Autore:
Itoh, H; Ueguchi-Tanaka, M; Sentoku, N; Kitano, H; Matsuoka, M; Kobayashi, M;
Indirizzi:
RIKEN, Tsukuba Inst, Tsukuba, Ibaraki 3050074, Japan RIKEN Tsukuba Ibaraki Japan 3050074 Inst, Tsukuba, Ibaraki 3050074, Japan Nagoya Univ, Biosci Ctr, Nagoya, Aichi 4648501, Japan Nagoya Univ Nagoya Aichi Japan 4648501 Ctr, Nagoya, Aichi 4648501, Japan Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648501, Japan Nagoya Univ Nagoya Aichi Japan 4648501 Sci, Nagoya, Aichi 4648501, Japan
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 15, volume: 98, anno: 2001,
pagine: 8909 - 8914
SICI:
0027-8424(20010717)98:15<8909:CAFAOT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZEA-MAYS-L; ORYZA-SATIVA; ENDOGENOUS GIBBERELLINS; INTERNODE LENGTH; ARABIDOPSIS-THALIANA; PHASEOLUS-VULGARIS; VEGETATIVE SHOOTS; SEEDS; METABOLISM; DWARF-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, M RIKEN, Tsukuba Inst, 3-1-1 Koyadal, Tsukuba, Ibaraki 3050074,Japan RIKEN 3-1-1 Koyadal Tsukuba Ibaraki Japan 3050074 0074, Japan
Citazione:
H. Itoh et al., "Cloning and functional analysis of two gibberellin 3 beta-hydroxylase genes that are differently expressed during the growth of rice", P NAS US, 98(15), 2001, pp. 8909-8914

Abstract

We have cloned two gibberellin (CA) 3 beta -hydroxylase genes, OsCA3ox1 and OsGA3ox2, from rice by screening a genomic library with a DNA fragment obtained by PCR using degenerate primers. We have used full-scan CC-MS and Kovats retention indices to show function for the two encoded recombinant fusion proteins. Both proteins show 3 beta -hydroxylase activity for the stepsGA(20) to GA(1), GA(5) to GA(3), GA(44) to GA(38) and GA(9) to GA(4). In addition, indirect evidence suggests that the OsGA3ox1 protein also has 2,3-desaturase activity, which catalyzes the steps GA(9) to 2,3-dehydro-GA(9) and GA(20) to GA(5) (2,3-dehydro GA(20)), and 2 beta -hydroxylase activity, which catalyzes the steps GA(1) to GA(8) and GA(4) to GA3(4) Molecular and linkage analysis maps the OsGA-ox1 gene to the distal end of the short arm of chromosome 5; the OsGA3ox2 gene maps to the distal end of the short arm of chromosome 1 that corresponds to the D18 locus. The association of the OsGA3ox2 gene with the d18 locus is confirmed by sequence and complementation analysis of three d18 alleles. Complementation of the d18-AD allele with the OxGA3ox2 gene results in transgenic plants with a normal phenotype. Although both genes show transient expression, the highest level for OsGA3ox1 is from unopened flower. The highest level for OsGA3ox2 is from elongating leaves.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:02:00