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Titolo:
Mechanism of DNA polymerase II-mediated frameshift mutagenesis
Autore:
Becherel, OJ; Fuchs, RPP;
Indirizzi:
Ecole Super Biotechnol Strasbourg, CNRS, UPR 9003, F-67400 Strasbourg, France Ecole Super Biotechnol Strasbourg Strasbourg France F-67400 ourg, France Univ Strasbourg, Inst Rech Canc Appareil Digest, CNRS, UPR 9003, F-67400 Strasbourg, France Univ Strasbourg Strasbourg France F-67400 03, F-67400 Strasbourg, France
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 15, volume: 98, anno: 2001,
pagine: 8566 - 8571
SICI:
0027-8424(20010717)98:15<8566:MODPIF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRRADIATED ESCHERICHIA-COLI; THYMINE DIMER BYPASS; MUTATION HOT-SPOT; CARCINOGEN N-2-ACETYLAMINOFLUORENE; TRANSLESION SYNTHESIS; REPETITIVE SEQUENCES; ADAPTIVE MUTATION; RECA PROTEIN; SOS RESPONSE; WILD-TYPE;
Keywords:
Narl mutation hot spot; translesion synthesis; slippage mutagenesis; N-2-acetylaminofluorene; umuDC (Pol V);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, RPP Ecole Super Biotechnol Strasbourg, CNRS, UPR 9003, Blvd Sebastien Brant, F-67400 Strasbourg, France Ecole Super Biotechnol Strasbourg BlvdSebastien Brant Strasbourg France F-67400
Citazione:
O.J. Becherel e R.P.P. Fuchs, "Mechanism of DNA polymerase II-mediated frameshift mutagenesis", P NAS US, 98(15), 2001, pp. 8566-8571

Abstract

Escherichia coil possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single N-2-acetylaminofluorene adduct within the Narl mutation hot spot. Indeed, error-free bypass requires Pol V (umuDC), whereas mutagenic (-2 frameshift) bypass depends on Pol II (polB), In this paper, we show that purified DNA Pol II is able in vitro to generate the -2 frameshift bypass product observed in vivo at the Narl sites, Although the Delta polB strain is completely defective in this mutation pathway, introduction of the polB gene on a low copy number plasmid restores the -2 frameshift pathway. Infact, modification of the relative copy number of polB versus umuDC genes results in a corresponding modification in the use of the frameshift versuserror-free translesion pathways, suggesting a direct competition between Pol II and V for the bypass of the same lesion, Whether such a polymerase competition model for translesion synthesis will prove to be generally applicable remains to be confirmed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:41:08