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Titolo:
Topological challenges to DNA replication: Conformations at the fork
Autore:
Postow, L; Crisona, NJ; Peter, BJ; Hardy, CD; Cozzarelli, NR;
Indirizzi:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 15, volume: 98, anno: 2001,
pagine: 8219 - 8226
SICI:
0027-8424(20010717)98:15<8219:TCTDRC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; LAMBDA INTEGRATIVE RECOMBINATION; ESCHERICHIA-COLI-CELLS; TOPOISOMERASE-IV; SUPERCOILED DNA; BACTERIOPHAGE-LAMBDA; IONIC CONDITIONS; PHAGE-MU; SV40 DNA; GYRASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Cozzarelli, NR Univ Calif Berkeley, Dept Mol & Cell Biol, 401 Barker Hall,Berkeley, CA 94720 USA Univ Calif Berkeley 401 Barker Hall Berkeley CA USA94720 A
Citazione:
L. Postow et al., "Topological challenges to DNA replication: Conformations at the fork", P NAS US, 98(15), 2001, pp. 8219-8226

Abstract

The unwinding of the parental DNA duplex during replication causes a positive linking number difference, or superhelical strain, to build up around the elongating replication fork. The branching at the fork and this strain bring about different conformations from that of (-) supercoiled DNA that isnot being replicated. The replicating DNA can form (+) precatenanes, in which the daughter DNAs are intertwined, and (+) supercoils. Topoisomerases have the essential role of relieving the superhelical strain by removing these structures. Stalled replication forks of molecules with a (f) superhelical strain have the additional option of regressing, forming a four-way junction at the replication fork. This four-way junction can be acted on by recombination enzymes to restart replication, Replication and chromosome folding are made easier by topological domain barriers, which sequester the substrates for topoisomerases into defined and concentrated regions. Domain barriers also allow replicated DNA to be (-) supercoiled. We discuss the importance of replicating DNA conformations and the roles of topoisomerases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 17:03:17