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Titolo:
Purification and identification of N-glycolylneuraminic acid (Neu5Gc) fromthe holothuroidea Gumi, Cucumaria echinata
Autore:
Sumi, T; Sallay, I; Asakawa, M; Park, SS; Miyazaki, M; Ohba, H;
Indirizzi:
Kyushu Natl Ind Res Inst, Tosu, Saga 8410052, Japan Kyushu Natl Ind Res Inst Tosu Saga Japan 8410052 osu, Saga 8410052, Japan Nacalai Tesque Inc, Kyoto 6170004, Japan Nacalai Tesque Inc Kyoto Japan 6170004 Tesque Inc, Kyoto 6170004, Japan Kumamoto Univ, Fac Educ, Food Sci Lab, Kumamoto 860555, Japan Kumamoto Univ Kumamoto Japan 860555 Food Sci Lab, Kumamoto 860555, Japan
Titolo Testata:
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
fascicolo: 2, volume: 31, anno: 2001,
pagine: 135 - 146
SICI:
1082-6068(2001)31:2<135:PAIONA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMATOGRAPHY MASS-SPECTROMETRY; STARFISH ASTERIAS-RUBENS; ACETYLNEURAMINIC ACID; SIALIC ACIDS; HUMAN-COLON; BIOSYNTHESIS; GANGLIOSIDES; HYDROXYLASE; ANTIGEN; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Ohba, H Kyushu Natl Ind Res Inst, 807-1 Shuku Machi, Tosu, Saga 8410052, Japan Kyushu Natl Ind Res Inst 807-1 Shuku Machi Tosu Saga Japan 8410052
Citazione:
T. Sumi et al., "Purification and identification of N-glycolylneuraminic acid (Neu5Gc) fromthe holothuroidea Gumi, Cucumaria echinata", PREP BIOC B, 31(2), 2001, pp. 135-146

Abstract

N-Glycolylneuraminic acid (Neu5Gc), precious sialic acid which could not be synthesized by a chemical method, occurrs in the body of holothuroidea, Gumi Cucumaria echinata. Gumi contains 85% of total sialic acid, as Neu5Gc, in the body. Neu5Gc was purified from dry powder of the body using Dowex 1-x8 (HCOO- form) anion exchange chromatography after mild acid hydrolysis with 0.1 N trifluoroacetic acid. Using GC-MS and H-1-NMR spectroscopy, the purified Neu5Gc was correctly identified to be Neu5Gc. The purity of Neu5Gc was more than 99%. This is the first report of purification and identification of Neu5Gc from holothuroidea by using anion exchange chromatography, GC-MS, and H-1-NMR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:51:24