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Titolo:
Rapid fractionation of bovine transferrin using immobilized gangliosides
Autore:
Walsh, MK; Nam, SH;
Indirizzi:
Utah State Univ, Dept Nutr & Food Sci, Logan, UT 84322 USA Utah State Univ Logan UT USA 84322 t Nutr & Food Sci, Logan, UT 84322 USA
Titolo Testata:
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
fascicolo: 2, volume: 31, anno: 2001,
pagine: 89 - 102
SICI:
1082-6068(2001)31:2<89:RFOBTU>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM TRANSFERRIN; ANION BINDING; CELL-SURFACE; LACTOFERRIN; RECEPTOR; PROTEINS; WHEY; MILK; PURIFICATION; MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Walsh, MK Utah State Univ, Dept Nutr & Food Sci, Logan, UT 84322 USA Utah State Univ Logan UT USA 84322 ood Sci, Logan, UT 84322 USA
Citazione:
M.K. Walsh e S.H. Nam, "Rapid fractionation of bovine transferrin using immobilized gangliosides", PREP BIOC B, 31(2), 2001, pp. 89-102

Abstract

Bovine transferrin (BTF) was fractionated from bovine whey using ganglioside affinity chromatography. After loading the immobilized matrix with a 2% whey solution, the matrix was washed with sodium acetate buffer at pH 4 containing 1 M NaCl before elution of BTF with sodium phosphate buffers at pH 7. Concanavalin-A affinity and ion exchange chromatography were used for further purification. The ganglioside column showed a 74.2% BTF recovery fromwhey and BTF was enriched to 61% purity with ion exchange chromatography. Bovine transferrin was identified by SDS-PAGE and western analysis. The Concanavalin-A affinity and ion exchange chromatography steps enriched BTF in the samples and removed other whey proteins from ganglioside purified fractions. These results indicate that immobilized ganglioside can be used to fractionate BTF from bovine whey. Our novel ganglioside affinity chromatography is rapid and efficient for the fractionation of BTF from whey.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 11:53:51