Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato
Autore:
Maeo, K; Tomiya, T; Hayashi, K; Akaike, M; Morikami, A; Ishiguro, S; Nakamura, K;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Dept Cellular Mechanisms & Funct, Biochem Lab,Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 5, volume: 46, anno: 2001,
pagine: 627 - 637
SICI:
0167-4412(2001)46:5<627:SEITPO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEAF-PETIOLE CUTTINGS; HIGH-LEVEL EXPRESSION; ARABIDOPSIS-THALIANA; INDUCIBLE EXPRESSION; SIGNAL-TRANSDUCTION; HIGHER-PLANTS; POLYGALACTURONIC ACID; PROTEIN-KINASES; NUCLEAR FACTOR; WD PROTEIN;
Keywords:
beta-amylase; cis element; gene expression; sporamin; sugar induction; transgenic tobacco;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamura, K Nagoya Univ, Grad Sch Bioagr Sci, Dept Cellular Mechanisms & Funct, Biochem Lab,Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 , Aichi 4648601, Japan
Citazione:
K. Maeo et al., "Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato", PLANT MOL B, 46(5), 2001, pp. 627-637

Abstract

Expression of genes coding for sporamin and beta -amylase, the two most abundant proteins in storage roots of sweet potato, is coordinately induciblein atypical vegetative tissues by sugars. A sweet potato gene for beta -amylase (beta -Amy) with introns as well as a beta -Amy::GUS fusion gene composed of the beta -Amy promoter and the GUS coding sequence, both showed sugar-inducible expression in leaves of transgenic tobacco which occurred via a hexokinase-independent pathway. Analyses using various 5'-terminal and internal deletions of the beta -Amy promoter indicated that truncated promoters of beta -Amy containing a sequence between -901 and -820, relative to the transcription start site, and the basic promoter region can confer sugar-inducible expression. This 82 bp region contained the TGGACGG sequence thatplays an essential role in the sugar-inducible expression of the truncatedpromoter of the sporamin gene. Deletion or base substitutions of this element in the truncated beta -Amy promoter abolished the sugar-inducible expression, the results suggesting that the TGGACGG element plays an important role in the coordinate induction of expression of genes for beta -amylase and sporamin by sugars.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 12:23:26