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Titolo:
Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro
Autore:
Sturaro, M; Viotti, A;
Indirizzi:
CNR, Ist Biosintesi Vegetali, I-20133 Milan, Italy CNR Milan Italy I-20133 R, Ist Biosintesi Vegetali, I-20133 Milan, Italy
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 5, volume: 46, anno: 2001,
pagine: 549 - 560
SICI:
0167-4412(2001)46:5<549:MOTOBI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL ACTIVATOR OPAQUE-2; REGULATORY LOCUS OPAQUE-2; ZEA-MAYS L; MAIZE ENDOSPERM; MOLECULAR ANALYSIS; TARGET SEQUENCES; PROTEIN; EXPRESSION; RECOGNIZES; MUTATION;
Keywords:
endosperm; maize; O2-box methylation; Opaque-2 bZIP; zein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Viotti, A CNR, Ist Biosintesi Vegetali, Via Bassini 15, I-20133 Milan, Italy CNR Via Bassini 15 Milan Italy I-20133 15, I-20133 Milan, Italy
Citazione:
M. Sturaro e A. Viotti, "Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro", PLANT MOL B, 46(5), 2001, pp. 549-560

Abstract

In the maize endosperm, the expression of the subfamily 4 (SF4) of the zein genes is under the transcriptional control of the Opaque2 (O2) basic leucine zipper transcriptional activator, which binds to the O2-box 5'-TCCACGTAGA-3'. Southern experiments showed that the O2-box core sequence ACGT is heavily methylated in sporophytic tissues but becomes hypomethylated in the endosperm. DNA analyses of two inbred lines and their reciprocal crosses indicate that the hypomethylation state is established on the maternal complements of the endosperm. Electrophoretic mobility shift assay (EMSA) and southwestern experiments with endosperm protein extracts and recombinant O2 using oligonucleotides with methylated and unmethylated cytosines in the O2-box indicate an inhibitory effect of modified sequences on O2-binding activity. These results suggest that DNA methylation modulates O2 activity in vivoand shed light on molecular mechanisms involved in the parent-dependent zein gene expression in maize endosperm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:40:40