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Titolo:
Changes in the mitochondrial proteome from mouse hearts deficient in creatine kinase
Autore:
Kernec, F; Unlu, M; Labeikovsky, W; Minden, JS; Koretsky, AP;
Indirizzi:
NINCDS, Lab Funct & Mol Imaging, Bethesda, MD 20892 USA NINCDS Bethesda MD USA 20892 Funct & Mol Imaging, Bethesda, MD 20892 USA Carnegie Mellon Univ, Dept Biol Sci, Natl Sci Fdn, Sci & Technol Ctr LightMicroscope Imaging & Biot, Pittsburgh, PA 15213 USA Carnegie Mellon Univ Pittsburgh PA USA 15213 ot, Pittsburgh, PA 15213 USA
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 2, volume: 6, anno: 2001,
pagine: 117 - 128
SICI:
1094-8341(200107)6:2<117:CITMPF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE-PHOSPHORYLATION; GEL-ELECTROPHORESIS; ENERGY HOMEOSTASIS; SKELETAL-MUSCLES; NITRIC-OXIDE; MICE; ISOENZYMES; ACONITASE; PROTEINS; IDENTIFICATION;
Keywords:
mitochondria; knockout mouse strain; two-dimensional electrophoresis; differential protein expression; protein map; matrix-assisted laser desorption/ionization mass spectrometry; difference gel electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Koretsky, AP NINCDS, Lab Funct & Mol Imaging, Rm 36-5B05,MSC 4159,36 Convent Dr, Bethesda, MD 20892 USA NINCDS Rm 36-5B05,MSC 4159,36 Convent Dr Bethesda MD USA 20892
Citazione:
F. Kernec et al., "Changes in the mitochondrial proteome from mouse hearts deficient in creatine kinase", PHYSIOL GEN, 6(2), 2001, pp. 117-128

Abstract

Creatine kinase (CK) is an abundant enzyme, important for maintenance of high-energy phosphate homeostasis in many tissues including heart. Double-knockout CK (DbKO-CK) mice missing both the muscle (MM) and sarcomeric mitochondrial (ScMit) isoforms of CK have recently been studied. Despite a large change in skeletal muscle function in DbKO-CK mice, there is little functional change in the heart. To investigate whether there are specific changes in cardiac mitochondrial proteins associated with the loss of MM- and ScMit-CK isoforms, we have used difference gel electrophoresis (DIGE) to comparemitochondrial proteins from wild-type and DbKO-CK mice. Mass spectrometry fingerprinting was used to identify 40 spots as known mitochondrial proteins. We have discovered that the loss of MM- and ScMit-CK isoforms did not cause large scale changes in heart mitochondrial proteins. The loss of ScMit-CK was readily detected in the DbKO-CK samples. We have also detected a large decrease in the precursor form of aconitase. Furthermore, two mitochondrial protein differences have been found in the parent mouse strains of the DbKO-CK mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:09:56