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Titolo:
Full-length cDNAs: more than just reaching the ends
Autore:
Das, M; Harvey, I; Chu, LL; Sinha, M; Pelletier, J;
Indirizzi:
McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 chem, Montreal, PQ H3G 1Y6, Canada McGill Univ, McGill Canc Ctr, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 Ctr, Montreal, PQ H3G 1Y6, Canada
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 2, volume: 6, anno: 2001,
pagine: 57 - 80
SICI:
1094-8341(200107)6:2<57:FCMTJR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; MURINE LEUKEMIA-VIRUS; TYPE-1 NUCLEOCAPSID PROTEIN; PRE-MESSENGER-RNA; HAMMERHEAD RIBOZYME CATALYSIS; AUG TRANSLATIONAL INITIATION; AVIAN-MYELOBLASTOSIS VIRUS; XENOPUS-LAEVIS OOCYTES; T7 DNA-POLYMERASE; REVERSE-TRANSCRIPTASE;
Keywords:
gene hunting; complementary DNA cloning; expressed sequence tags; expression validated gene;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
167
Recensione:
Indirizzi per estratti:
Indirizzo: Pelletier, J McGill Univ, Dept Biochem, McIntyre Med Sci Bldg,Rm 810,3655 Drummond St, Montreal, PQ H3G 1Y6, Canada McGill Univ McIntyre Med Sci Bldg,Rm 810,3655 Drummond St Montreal PQ Canada H3G 1Y6
Citazione:
M. Das et al., "Full-length cDNAs: more than just reaching the ends", PHYSIOL GEN, 6(2), 2001, pp. 57-80

Abstract

The development of functional genomic resources is essential to understandand utilize information generated from genome sequencing projects. Centralto the development of this technology is the creation of high-quality cDNAresources and improved technologies for analyzing coding and noncoding mRNA sequences. The isolation and mapping of cDNAs is an entree to characterizing the information that is of significant biological relevance in the genome of an organism. However, a bottleneck is often encountered when attempting to bring to full-length (or at least full-coding) a number of incompletecDNAs in parallel, since this involves the nonsystematic, time consuming, and labor-intensive iterative screening of a number of cDNA libraries of variable quality and/or directed strategies to process individual clones (e.g., 5' rapid amplification of cDNA ends). Here, we review the current state of the art in cDNA library generation, as well as present an analysis of the different steps involved in cDNA library generation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:05:57