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Titolo:
Extracellular carbonic anhydrase in the dogfish, Squalus acanthias: A rolein CO2 excretion
Autore:
Gilmour, KM; Perry, SF; Bernier, NJ; Henry, RP; Wood, CM;
Indirizzi:
Bamfield Marine Stn, Bamfield, BC V0R 1B0, Canada Bamfield Marine Stn Bamfield BC Canada V0R 1B0 mfield, BC V0R 1B0, Canada Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada Carleton Univ OttawaON Canada K1S 5B6 t Biol, Ottawa, ON K1S 5B6, Canada Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada Univ Ottawa Ottawa ON Canada K1N 6N5 ept Biol, Ottawa, ON K1N 6N5, Canada Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada Univ Alberta Edmonton AB Canada T6G 2E9 Sci, Edmonton, AB T6G 2E9, Canada Auburn Univ, Dept Zool, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 urn Univ, Dept Zool, Auburn, AL 36849 USA McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada McMaster Univ Hamilton ON Canada L8S 4K1 ol, Hamilton, ON L8S 4K1, Canada
Titolo Testata:
PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY
fascicolo: 4, volume: 74, anno: 2001,
pagine: 477 - 492
SICI:
1522-2152(200107/08)74:4<477:ECAITD>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-BASE DISEQUILIBRIUM; SKATE RAJA-OCELLATA; AIR-BREATHING FISH; ISOLATED RAT LUNGS; BOWFIN AMIA-CALVA; RED-BLOOD-CELL; DIOXIDE EXCRETION; SPINY DOGFISH; RAINBOW-TROUT; ELASMOBRANCH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Gilmour, KM Bamfield Marine Stn, Bamfield, BC V0R 1B0, Canada Bamfield Marine Stn Bamfield BC Canada V0R 1B0 0R 1B0, Canada
Citazione:
K.M. Gilmour et al., "Extracellular carbonic anhydrase in the dogfish, Squalus acanthias: A rolein CO2 excretion", PHYSIOL B Z, 74(4), 2001, pp. 477-492

Abstract

In Pacific spiny dogfish (Squalus acanthias), plasma CO2 reactions have access to plasma carbonic anhydrase (CA) and gill membrane-associated CA. Theobjectives of this study were to characterise the gill membrane-bound CA and investigate whether extracellular CA contributes significantly to CO2 excretion in dogfish. A subcellular fraction containing membrane-associated CA activity was isolated from dogfish gills and incubated with phosphatidylinositol-specific phospholipase C. This treatment caused significant releaseof CA activity from its membrane association, a result consistent with identification of the dogfish gill membrane-bound CA as a type IV isozyme. Inhibition constants (K-i) against acetazolamide and benzolamide were 4.2 and 3.5 nmol L-1, respectively. Use of a low dose (1.3 mg kg(-1) or 13 mu mol L-1) of benzolamide to selectively inhibit extracellular CA in vivo caused asignificant 30%-60% reduction in the arterial-venous total CO2 concentration difference, a significant increase in Pco(2) and an acidosis, without affecting blood flow or ventilation. No effect of benzolamide on any measure of CO2 excretion was detected in rainbow trout (Oncorhynchus mykiss). Theseresults indicate that extracellular CA contributes substantially to CO2 excretion in the dogfish, an elasmobranch, and confirm that CA is not available to plasma CO2 reactions in rainbow trout, a teleost.

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Documento generato il 04/04/20 alle ore 15:21:21