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Titolo:
Inhibition of enteroaggregative Escherichia coli adhesion to HEp-2 cells by secretory immunoglobulin A from human colostrum
Autore:
Fernandes, RM; Carbonare, SB; Carneiro-Sampaio, MMS; Trabulsi, LR;
Indirizzi:
Inst Butantan, Lab Imunogenet, BR-05503900 Sao Paulo, Brazil Inst Butantan Sao Paulo Brazil BR-05503900 BC-05503900 Sao Paulo, Brazil Butantan Inst, Special Lab Microbiol, Sao Paulo, Brazil Butantan Inst SaoPaulo Brazil Special Lab Microbiol, Sao Paulo, Brazil Univ Fed Santa Maria, Dept Microbiol & Parasitol, BR-97119900 Santa Maria,RS, Brazil Univ Fed Santa Maria Santa Maria RS Brazil BR-97119900 BCMaria,RS, Brazil Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil ed Sci, Dept Immunol, Sao Paulo, Brazil
Titolo Testata:
PEDIATRIC INFECTIOUS DISEASE JOURNAL
fascicolo: 7, volume: 20, anno: 2001,
pagine: 672 - 678
SICI:
0891-3668(200107)20:7<672:IOEECA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGGREGATIVE ADHERENCE; PERSISTENT DIARRHEA; INTESTINAL-MUCOSA; HELA-CELLS; IN-VITRO; STRAINS; ENTEROTOXIN; CHILDREN; PROTEINS; PATTERNS;
Keywords:
enteroaggregative Escherichia coli; infantile diarrhea; human colostrum; bacterial adhesion; secretory IgA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Carbonare, SB Inst Butantan, Lab Imunogenet, Av Dr Vital Brasil 1500, BR-05503900 Sao Paulo, Brazil Inst Butantan Av Dr Vital Brasil 1500 Sao Paulo Brazil BR-05503900 BC
Citazione:
R.M. Fernandes et al., "Inhibition of enteroaggregative Escherichia coli adhesion to HEp-2 cells by secretory immunoglobulin A from human colostrum", PEDIAT INF, 20(7), 2001, pp. 672-678

Abstract

Background. Enteroaggregative Escherichia coli (EAEC) is an important agent of the persistent diarrhea among low socioeconomic level children in developing countries that may be associated with chronic undernourishment, Breast-feeding is effective in protecting infants against diarrhea and other infectious diseases. The aim of the study is to verify the ability of human colostrum to inhibit aggregative adhesion of EAEC to HEp-2 cells and the presence of antibodies reactive to antigenic fractions of EAEC in colostrum samples. Methods. Enzyme-linked immunosorbent assay, immunoblotting and adhesion assays of EAEC to HEp-2 cells were done with pooled or individual colostrum samples (n = 35), Assays were performed with a well-known EAEC strain, 044:H18 E, coli (strain 042), Colostral IgA was isolated by affinity chromatography in Sepharose anti-human alpha chain column. Results. Total colostrum and isolated IgA inhibited EAEC adhesion, and this ability was associated with the presence of IgA antibodies against a 15-kDa band, compatible with the subunits of aggregative adherence fimbrial adhesin II, characteristic of the 042 strain, absent in its plasmid-cured isogenic strain, that was used as control. Individual colostrum samples also inhibited adhesion, showed variable antibody titles against EAEC antigens in enzyme-linked immunosorbent assay and recognized many antigenic fractions in immunoblotting assays, including the 15-kDa band. Conclusions. These results confirm that IgA from human colostrum inhibits adhesion of EAEC to HEp-2 cells and suggest that colostrum IgA antibodies reactive to EAEC antigens may play a role in protection of infants against diarrhea caused by these bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:54:43