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Titolo:
Prevalence and rapid identification of clarithromycin-resistant Helicobacter pylori isolates in children
Autore:
Yang, YJ; Yang, JC; Jeng, YM; Chang, MH; Ni, YH;
Indirizzi:
Natl Taiwan Univ, Coll Med & Hosp, Dept Pediat, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 , Dept Pediat, Taipei 10764, Taiwan Natl Taiwan Univ, Coll Med & Hosp, Dept Internal Med, Taipei 10764, TaiwanNatl Taiwan Univ Taipei Taiwan 10764 Internal Med, Taipei 10764, Taiwan Natl Taiwan Univ, Coll Med & Hosp, Dept Pathol, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 , Dept Pathol, Taipei 10764, Taiwan
Titolo Testata:
PEDIATRIC INFECTIOUS DISEASE JOURNAL
fascicolo: 7, volume: 20, anno: 2001,
pagine: 662 - 666
SICI:
0891-3668(200107)20:7<662:PARIOC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
23S RIBOSOMAL-RNA; TRIPLE THERAPY; MACROLIDE RESISTANCE; POINT MUTATIONS; METRONIDAZOLE; GASTRITIS; DISEASE; GENE;
Keywords:
Helicobacter pylori; antibiotic resistance; clarithromycin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Ni, YH Natl Taiwan Univ Hosp, Dept Pediat, 7 Chung Shan S Rd, Taipei 100, Taiwan Natl Taiwan Univ Hosp 7 Chung Shan S Rd Taipei Taiwan 100 , Taiwan
Citazione:
Y.J. Yang et al., "Prevalence and rapid identification of clarithromycin-resistant Helicobacter pylori isolates in children", PEDIAT INF, 20(7), 2001, pp. 662-666

Abstract

Background. Little is known about the prevalence of antibiotic-resistant Helicobacter pylori infection in children. Culture and antimicrobial susceptibility testing are generally time-consuming and not a routine in many hospitals. Objective. To investigate the prevalence of clarithromycin-resistant H. pylori strains in children, to identify those isolates via rapid methodology and to examine the severity of gastritis caused by the antibiotic-resistantH. pylori isolates. Methods. Enrolled were 245 children investigated for H, pylori infection by endoscopic examination. The gastric antral specimens were subjected to DNA extraction and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with primers specific to the H. pylori 23S rRNA gene. Conventional bacterial cultures were performed simultaneously as the diagnostic standard. Minimal inhibitory concentrations of clarithromycin and metronidazole were determined by E test. This was used as a standard to determine the sensitivity and specificity of the above PCR-RFLP assay. The specimens were processed for histologic examination and evaluated by the updated Sydney system. Results. H. pylori was isolated in 67 of the 245 children; 12 (18%) of them were clarithromycin-resistant and 6 (9%) were metronidazole-resistant. Nodifference in histologic examinations was noted between the antibiotic-resistant and -susceptible strains. We performed PCR-RFLP with all 12 clarithromycin-resistant isolates: 10 had a 23S ribosomal RNA A2144G point mutation; 1 had a mixture of an A2143G point mutant and susceptible strains; and 1 had neither of the 2 mutations. Conclusions. The prevalence of clarithromycin-resistant H. pylori isolatesin Taiwanese children is 18%. PCR-RFLP had a high sensitivity (92%) and specificity (100%) for the clarithromycin resistance gene mutation determination. The dominant mutation is A2144G. PCR-RFLP provides a rapid and accurate approach to detect clarithromycin-resistant strains within 24 h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:06:44