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Titolo:
The use of a synthetic antigen for the serological diagnosis of human trichinellosis
Autore:
Bruschi, F; Moretti, A; Wassom, D; Fioretti, DP;
Indirizzi:
Univ Pisa, BMIE, Dipartimento Patol Sperimentale, I-56126 Pisa, Italy UnivPisa Pisa Italy I-56126 nto Patol Sperimentale, I-56126 Pisa, Italy Univ Perugia, Dipartimento Sci Biopatol Vet, I-06100 Perugia, Italy Univ Perugia Perugia Italy I-06100 Biopatol Vet, I-06100 Perugia, Italy Heska Corp, Ft Collins, CO USA Heska Corp Ft Collins CO USAHeska Corp, Ft Collins, CO USA
Titolo Testata:
PARASITE-JOURNAL DE LA SOCIETE FRANCAISE DE PARASITOLOGIE
fascicolo: 2, volume: 8, anno: 2001, supplemento:, S
pagine: S141 - S143
SICI:
1252-607X(200106)8:2<S141:TUOASA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBOHYDRATE-ASSOCIATED DETERMINANT; SPIRALIS ANTIGENS; ANTIBODY-RESPONSE; IMMUNODOMINANT;
Keywords:
beta-tyvelose; human trichinellosis; IgG; IgE; diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Bruschi, F Univ Pisa, BMIE, Dipartimento Patol Sperimentale, Via Roma 55, I-56126 Pisa, Italy Univ Pisa Via Roma 55 Pisa Italy I-56126 , I-56126 Pisa, Italy
Citazione:
F. Bruschi et al., "The use of a synthetic antigen for the serological diagnosis of human trichinellosis", PARASITE, 8(2), 2001, pp. S141-S143

Abstract

Hosts infected with Trichinella produce antibodies specific for an epitopecommon to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verifythe specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using beta -tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were used. In trichinellosis patient sera, specific IgG were present in 100 % of cases, irrespective of the antigen used, but whereas specific IgE were detected in 78 % using E/S antigens, a 100% positivityrate was obtained, using the beta -tyvelose- BSA conjugate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:38:27