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Titolo:
Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis
Autore:
Obexer, P; Certa, U; Kofler, R; Helmberg, A;
Indirizzi:
Innsbruck Univ, Sch Med, Inst Pathophysiol, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 ysiol, A-6020 Innsbruck, Austria F Hoffmann La Roche & Co Ltd, Roche Genet, CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4070 asel, Switzerland
Titolo Testata:
ONCOGENE
fascicolo: 32, volume: 20, anno: 2001,
pagine: 4324 - 4336
SICI:
0950-9232(20010719)20:32<4324:EPOGTC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; KAPPA-B ACTIVITY; INDUCED APOPTOSIS; FK506-BINDING IMMUNOPHILIN; TRANSCRIPTIONAL ACTIVATION; REGULATED APOPTOSIS; DOWN-REGULATION; COMPLETE CDNA; DNA-BINDING; G1 ARREST;
Keywords:
chemotherapy; gene chip; glucocorticoid; leukemia; pathophysiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Helmberg, A Innsbruck Univ, Sch Med, Inst Pathophysiol, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 0 Innsbruck, Austria
Citazione:
P. Obexer et al., "Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis", ONCOGENE, 20(32), 2001, pp. 4324-4336

Abstract

To arrive at a better understanding of the effects of the glucocorticoid component of chemotherapy protocols on lymphocytic leukemia cells, we analysed early responses of T-lymphocytic leukemia cell lines Jurkat and CEM-C7, both of which undergo apoptosis in response to dexamethasone, via gene chips. Among genes identified as repressed, a notable cluster seemed to be of importance for the processes of transcription, mRNA splicing and protein synthesis. Consequently, we assessed time-resolved uptake of uridine and methionine to monitor RNA and protein synthesis, along with parameters quantifying apoptosis, Repression of uptake to about 65% of that in untreated cells preceded the first sign of apoptosis by several hours in both cell lines, In addition to this general repression of RNA and protein synthesis, severalgenes were found to be regulated that may contribute to synergistic actionof glucocorticoids with other components of frequently used chemotherapy protocols such as antimetabolites, methotrexate and alkylating agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 14:20:33