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Titolo:
The production and evaluation of antibodies for enzyme immunoassay of AZTTP
Autore:
Akeb, F; Creminon, C; Grassi, J; Guedj, R; Duval, D;
Indirizzi:
Univ Nice, CNRS, Chim Bioorgan Lab, ESA 6001,Fac Sci, F-06108 Nice 2, France Univ Nice Nice France 2 an Lab, ESA 6001,Fac Sci, F-06108 Nice 2, France CEA Saclay, Serv Pharmacol & Immunol, Direct Sci Vivant, F-91191 Gif Sur Yvette, France CEA Saclay Gif Sur Yvette France F-91191 F-91191 Gif Sur Yvette, France
Titolo Testata:
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
fascicolo: 3, volume: 20, anno: 2001,
pagine: 243 - 250
SICI:
1525-7770(2001)20:3<243:TPAEOA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; BLOOD MONONUCLEAR-CELLS; ZIDOVUDINE PHOSPHORYLATION; INFECTED PATIENTS; 3'-AZIDO-3'-DEOXYTHYMIDINE AZT; INTRACELLULAR PHOSPHORYLATION; CARTRIDGE RADIOIMMUNOASSAY; REVERSE-TRANSCRIPTASE; PLASMA-CONCENTRATIONS; COMPETITIVE ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Duval, D Univ Nice, CNRS, Chim Bioorgan Lab, ESA 6001,Fac Sci, Parc Valrose, F-06108 Nice 2, France Univ Nice Parc Valrose Nice France 2 ose, F-06108 Nice 2, France
Citazione:
F. Akeb et al., "The production and evaluation of antibodies for enzyme immunoassay of AZTTP", NUCLEOS NUC, 20(3), 2001, pp. 243-250

Abstract

We describe the development of the first enzyme immunoassay for quantifying AZTTP that does not use of radioactive labeling. Anti-AZTTP antibodies were raised in rabbits by immunizing with an AZTTP-kelhoyle limpet hemocyanin(KLH) conjugate. Competitive immunoassays indicated a nanomolar sensitivity to AZTTP. One of the antisera produced was specific for AZTTP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:50:40