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Titolo:
Translesion synthesis by yeast DNA polymerase zeta from templates containing lesions of ultraviolet radiation and acetylaminofluorene
Autore:
Guo, DY; Wu, XH; Rajpal, DK; Taylor, JS; Wang, ZG;
Indirizzi:
Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 Ctr Toxicol, Lexington, KY 40536 USA Washington Univ, Dept Chem, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 , Dept Chem, St Louis, MO 63130 USA
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 13, volume: 29, anno: 2001,
pagine: 2875 - 2883
SICI:
0305-1048(20010701)29:13<2875:TSBYDP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMINE-THYMINE DIMER; RAD18 GENE-PRODUCT; SACCHAROMYCES-CEREVISIAE; XERODERMA-PIGMENTOSUM; EMBRYONIC LETHALITY; INDUCED MUTAGENESIS; CATALYTIC SUBUNIT; POSTREPLICATION REPAIR; HUMAN HOMOLOG; ERROR-FREE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, ZG Univ Kentucky, Grad Ctr Toxicol, 306 Hlth Sci Res Bldg, Lexington, KY 40536 USA Univ Kentucky 306 Hlth Sci Res Bldg Lexington KY USA 40536 36 USA
Citazione:
D.Y. Guo et al., "Translesion synthesis by yeast DNA polymerase zeta from templates containing lesions of ultraviolet radiation and acetylaminofluorene", NUCL ACID R, 29(13), 2001, pp. 2875-2883

Abstract

In the yeast Saccharomyces cerevisiae, DNA polymerase zeta (Pol zeta) is required in a major lesion bypass pathway. To help understand the role of Pol zeta in lesion bypass, we have performed in vitro biochemical analyses ofthis polymerase in response to several DNA lesions. Purified yeast Pol zeta performed limited translesion synthesis opposite a template TT (6-4) photoproduct, incorporating A or T with similar efficiencies land less frequently G) opposite the 3 ' T, and predominantly A opposite the 5 ' T, Purified yeast Pol zeta predominantly incorporated a G opposite an acetylaminofluorene (AAF)-adducted guanine, The lesion, however, significantly inhibited subsequent extension. Furthermore, yeast Pol zeta catalyzed extension DNA synthesis from primers annealed opposite the AAF-guanine and the 3 ' T of the TT (6-4) photoproduct with varying efficiencies. Extension synthesis was more efficient when A or C was opposite the AAF-guanine, and when G was opposite the 3 ' T of the TT (6-4) photoproduct. In contrast, the 3 ' T of a cis-syn TT dimer completely blocked purified yeast Pol zeta, whereas the 5 ' T was readily bypassed. These results support the following dual-function model of Pol zeta. First, Pol zeta catalyzes nucleotide incorporation oppositeAAF-guanine and TT (6-4) photoproduct with a limited efficiency. Secondly,more efficient bypass of these lesions may require nucleotide incorporation by other DNA polymerases followed by extension DNA synthesis by Pol zeta.

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Documento generato il 02/04/20 alle ore 09:30:18