Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Translesional synthesis on DNA templates containing the 2 '-deoxyribonolactone lesion
Autore:
Berthet, N; Roupioz, Y; Constant, JF; Kotera, M; Lhomme, J;
Indirizzi:
Univ Grenoble 1, UMR CNRS 5616, LEDSS, F-38041 Grenoble 9, France Univ Grenoble 1 Grenoble France 9 616, LEDSS, F-38041 Grenoble 9, France
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 13, volume: 29, anno: 2001,
pagine: 2725 - 2732
SICI:
0305-1048(20010701)29:13<2725:TSODTC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENT SEQUENCE CONTEXTS; ABASIC SITES; POLYMERASE-I; A-RULE; 2-DEOXYRIBONOLACTONE-CONTAINING NUCLEOTIDE; BETA-ELIMINATION; STRAND SCISSION; MECHANISM; NEOCARZINOSTATIN; DAMAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Berthet, N Univ Grenoble 1, UMR CNRS 5616, LEDSS, BP 53, F-38041 Grenoble 9, France Univ Grenoble 1 BP 53 Grenoble France 9 041 Grenoble 9, France
Citazione:
N. Berthet et al., "Translesional synthesis on DNA templates containing the 2 '-deoxyribonolactone lesion", NUCL ACID R, 29(13), 2001, pp. 2725-2732

Abstract

A site-specifically modified oligonucleotide containing a single 2 ' -deoxyribonolactone lesion was used as a template for primer extension reactionscatalyzed by M-MuLV reverse transcriptase (RT) and by the Klenow fragmentsof Escherichia coli DNA polymerase proficient (KF exo(+)) or deficient (KFexo(-)) in exonuclease activity. Analysis of the extension products in thepresence of the four dNTPs or of a single dNTP showed that the M-MuLV RT was completely blocked and did not incorporate any dNMP opposite 2 ' -deoxyribonolactone. KF exo(-) preferentially incorporated nucleotides opposite the lesion following the frequency order dAMP > dGMP much greater than dTMP similar to dCMP and thus appeared to obey the 'A rule' for preferential incorporation as has been shown previously for the 2 ' -deoxyribose abasic site. In the sequence context examined, the primer extension by KF exo- appeared to be less efficient when dAMP was positioned opposite the lesion as compared with dTMP or dGMP, These two nucleotides promoted a more efficient polymerization accompanied by nucleotide deletion through misalignment incorporations. We therefore predict that the sequence context may strongly influence the translesional synthesis by KF exo(-) and thus the miscoding and mutational potential of the 2 ' -deoxyribonolactone in E. coli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:22:43