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Titolo:
Strong depletion of CD14(+)CD16(+) monocytes during haemodialysis treatment
Autore:
Sester, U; Sester, M; Heine, G; Kaul, H; Girndt, M; Kohler, H;
Indirizzi:
Univ Saarland, Dept Med 4, D-6650 Homburg, Germany Univ Saarland HomburgGermany D-6650 Dept Med 4, D-6650 Homburg, Germany
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 7, volume: 16, anno: 2001,
pagine: 1402 - 1408
SICI:
0931-0509(200107)16:7<1402:SDOCMD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC-RENAL-FAILURE; NECROSIS-FACTOR-ALPHA; HEMODIALYSIS-PATIENTS; COMPLEMENT ACTIVATION; GENE-EXPRESSION; LONG-TERM; INTERLEUKIN-6; SUBPOPULATION; MORTALITY; MEMBRANES;
Keywords:
CD14(+)CD16(+) monocytes; flow cytometry; haemodialysis; inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kohler, H Univ Homburg, Dept Med 4, D-66421 Homburg, Germany Univ HomburgHomburg Germany D-66421 D-66421 Homburg, Germany
Citazione:
U. Sester et al., "Strong depletion of CD14(+)CD16(+) monocytes during haemodialysis treatment", NEPH DIAL T, 16(7), 2001, pp. 1402-1408

Abstract

Background. The immune defect in haemodialysis (HD) patients is associate with a monocytic dysfunction, including an increased production of proinflammatory cytokines. Monocytes fall into subpopulations comprising CD14(++)CD(-) and CD(+)CD16(+) cells. Circulating numbers of the latter can rapidly increase during infectious episodes and inflammation. Methods. We determined the amount of CD14(+)CD16(+) monocytes in HD patients and characterized their fate during HD treatment. In 34 HD patients and 17 healthy controls, the distinct cell populations were determined by differential blood counts and flow cytometry. Cells from 14 HD patients were analysed at the start, 10, 30 and 120 min thereafter, and at the end of HD treatment. Results. Before HD, patients show a monocytosis with a strongly increased CD14(+)CD16(+) subpopulation. Early during HD treatment, circulating leukocyte numbers decrease, with monocytes being most profoundly influenced. Interestingly, among them, sequestration is most pronounced in the CD14(+)CD16() subpopulation. After 30 min. similar to 83 +/-9% of CD14(+)CD16(+) cellswere removed from circulation. This sequestration does not differ between patients treated with polyamide or haemophan membranes. The sequestration is a short-lived temporary effect and cell numbers are replenished within 120 min of treatment for the entire monocyte population. Beyond that time point, cellular activation by the dialyser membrane becomes visible. Reappearance kinetics of CD14(+)CD16(+) monocytes is slower; however, initial numbers are reached by the end of treatment. Conclusion . Haemodiaysis leads to temporary removal of monocytes from thebloodstream followed by the reappearance of activated cells. This might contribute to the state of chronic microinflammation, which is reflected by high levels of CD14(+)CD16(+) monocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:18:02