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Titolo:
Formation of DNA adducts in the aorta of smoke-exposed rats, and modulation by chemopreventive agents
Autore:
Izzotti, A; Camoirano, A; Cartiglia, C; Tampa, E; De Flora, S;
Indirizzi:
Univ Genoa, Dept Hlth Sci, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 enoa, Dept Hlth Sci, I-16132 Genoa, Italy
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 494, anno: 2001,
pagine: 97 - 106
SICI:
1383-5718(20010725)494:1-2<97:FODAIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYLCYSTEINE; CIGARETTE-SMOKE; ATHEROSCLEROSIS; INHIBITION; INDUCTION; CANCER; PROLIFERATION; MECHANISMS; BIOMARKERS; MUTATIONS;
Keywords:
cigarette smoke; aorta; DNA adducts; atherosclerosis; chemoprevention;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: De Flora, S Univ Genoa, Dept Hlth Sci, Via Pastore 1, I-16132 Genoa, ItalyUniv Genoa Via Pastore 1 Genoa Italy I-16132 132 Genoa, Italy
Citazione:
A. Izzotti et al., "Formation of DNA adducts in the aorta of smoke-exposed rats, and modulation by chemopreventive agents", MUT RES-GTE, 494(1-2), 2001, pp. 97-106

Abstract

Our previous studies showed that nucleotide alterations, evaluated by P-32postlabeling, are systematically detected in smooth muscle cells of atherosclerotic lesions localized in the aorta of surgical patients. The level ofthese molecular lesions was correlated with the occurrence of known atherogenic risk factors, among which the number of currently smoked cigarettes, and was significantly enhanced in individuals having a null GSTM1 genotype as compared to individuals carrying the GSTM1 genotype. The present study had the dual objective of evaluating the formation of DNA adducts in the whole thoracic aorta of Sprague-Dawley rats, exposed whole-body to cigarette smoke for 28 consecutive days, and of investigating the effects of chemopreventive agents given orally during the same period. High levels of P-32 postlabeled DNA adducts were formed in the aorta of smoke-exposed rats, with anoverall 11 times increase over the total levels observed in sham-exposed rats, and with increases ranging between three and 63 times for seven individual DNA adducts. Supplement of the diet with either 1,2-dithiole-3-thione,phenethyl isothiocyanate or 5,6-benzoflavone had no or poor effects on thesmoke-related formation of nucleotide alterations in the aorta. In contrast, oltipraz, given with the diet, N-acetyl-L-cysteine. given with drinking water and, even more potently, their combination exerted remarkable protective effects. The results of this experimental study, together with the previous findings in humans, suggest that DNA alterations may contribute to theatherogenic process, clarify a possible mechanism of cigarette smoke, a well known atherogen, and show the potential protective effects of certain drugs towards these alterations. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:02:03