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Titolo:
Time- and dose-dependent DNA binding of PAHs derived from diesel particle extracts, benzo[a]pyrene and 5-methylchrysene in a human mammary carcinoma cell line (MCF-7)
Autore:
Kuljukka-Rabb, T; Peltonen, K; Isotalo, S; Mikkonen, S; Rantanen, L; Savela, K;
Indirizzi:
FIOH, FIN-00250 Helsinki, Finland FIOH Helsinki Finland FIN-00250FIOH, FIN-00250 Helsinki, Finland Fortum Oil & Gas Oy, Porvoo, Finland Fortum Oil & Gas Oy Porvoo FinlandFortum Oil & Gas Oy, Porvoo, Finland
Titolo Testata:
MUTAGENESIS
fascicolo: 4, volume: 16, anno: 2001,
pagine: 353 - 358
SICI:
0267-8357(200107)16:4<353:TADDBO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; GLUTATHIONE-S-TRANSFERASE; METABOLIC-ACTIVATION; MOUSE SKIN; EXCISION-REPAIR; DIOL EPOXIDES; CARBON-BLACK; IN-VITRO; ADDUCTS; EXHAUST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Savela, K FIOH, Topeliuksenkatu 41B, FIN-00250 Helsinki, Finland FIOH Topeliuksenkatu 41B Helsinki Finland FIN-00250 ki, Finland
Citazione:
T. Kuljukka-Rabb et al., "Time- and dose-dependent DNA binding of PAHs derived from diesel particle extracts, benzo[a]pyrene and 5-methylchrysene in a human mammary carcinoma cell line (MCF-7)", MUTAGENESIS, 16(4), 2001, pp. 353-358

Abstract

Cultures of a human mammary carcinoma cell line (MCF-7) were exposed to the soluble organic fraction of diesel particle emissions, benzo[a]pyrene (B[a]P) and 5-methyl-chrysene (5-MeCHR) to study time- and dose-related PAH-DNA binding, The concentrations of 14 PAHs in three extracts were analyzed byHPLC and PAH-DNA adducts were measured by P-32 post-labeling assay. Time-dependent DNA adducts formation of 2.5 muM B[a]P was lower than that of 2.5 muM 5-MeCHR. In comparison with B[a]P, 2-fold higher adduct formation by 5-MeCHR was observed at 12 h exposure, after which BPDE adducts decreased and5-MeCHR continued to form adducts linearly during 48 h exposure. The data for these two PAH compounds demonstrate a large variation in adduct-formingpotency, which should be taken into account when estimating DNA adducts formed by mixtures of unknown PAHs, A clear dose-response effect on formationof DNA adducts was obtained for B[a]P and a Standard Reference Material (SRM) of diesel particulate matter. The amount of B[a]P contributed more to total DNA adduct formation by SRM than by three diesel extracts. Thus, no conclusions can be drawn from diesel particle-derived Bla]P as to the adduct-forming potency of other carcinogenic PAHs, There was little change in adduct levels formed by three diesel extracts from 0 to 12 h exposure. Thereafter, the number of adducts formed by RD2 increased more rapidly than those formed by RD1 and EN97. The concentrations of 14 PAHs and adduct levels analyzed at 24 and 48 h did not change in the same proportion between the extracts. Neither could PAH-DNA adduct levels be explained by the sum of strong and weak adduct-forming PAHs analyzed in the extracts. This indicates that other PAHs in the extracts RD1, RD2 and EN97 contributed to adduct formation more than the carcinogenic adduct-forming PAHs analyzed in this study.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:14:23