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Titolo:
Effects of linear polyethylenimine and polyethylenimine/DNA on lung function after airway instillation to rat lungs
Autore:
Uduehi, AN; Stammberger, U; Kubisa, B; Gugger, M; Buehler, TA; Schmid, RA;
Indirizzi:
Univ Hosp Bern, Div Gen Thorac Surg, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 Surg, CH-3010 Bern, Switzerland Univ Hosp Bern, Dept Pathol, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 athol, CH-3010 Bern, Switzerland Novartis, Transplantat Biol, Basel, Switzerland Novartis Basel Switzerland artis, Transplantat Biol, Basel, Switzerland
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 4, anno: 2001,
pagine: 52 - 57
SICI:
1525-0016(200107)4:1<52:EOLPAP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; IN-VIVO; CARDIAC ALLOGRAFTS; AEROSOL DELIVERY; MOUSE LUNG; CPG MOTIFS; DNA; EXPRESSION; SURVIVAL; CTLA4IG;
Keywords:
polyethylenimine; non-viral gene delivery; lung; airway instillation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Schmid, RA Univ Hosp Bern, Div Gen Thorac Surg, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 010 Bern, Switzerland
Citazione:
A.N. Uduehi et al., "Effects of linear polyethylenimine and polyethylenimine/DNA on lung function after airway instillation to rat lungs", MOL THER, 4(1), 2001, pp. 52-57

Abstract

Lung transplantation is an acceptable treatment option for various end-stage pulmonary diseases, but long-term survival currently lags behind that after transplantation of other solid organs. We hypothesized that gene transfer to grafts before transplantation may be a useful method to deliver antioxidant and/or anti-inflammatory genes to modulate these processes. For thispurpose, we assessed the efficiency of gene transfer and effects on lung function of the synthetic polycation, linear polyethylenimine (PEI), after airway instillation to the lungs of Fischer rats. Twenty-four hours after gene delivery, reporter gene activity in DNA/PEI treated rats was approximately 12-fold higher than that in rats treated with naked DNA, but by 72 hoursthere was no significant difference between groups and activity had decreased by at least 85%. Function of the transfected left lung was assessed by measuring arterial PaO2 levels and was found to be significantly lower at 24 and 72 hours after gene transfer in the PEI/DNA group compared with the naked DNA group. The deterioration in lung function correlated with histological findings. Rats treated with PEI alone and sacrificed after 72 hours showed an impairment in lung function similar to tha seen with PEI/DNA treatment. Our studies highlight the importance of assessing the functional capacity of a graft after gene transfer to determine suitability for subsequent transplantation.

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Documento generato il 29/02/20 alle ore 08:21:25