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Titolo:
Dark green islands in plant virus infection are the result of posttranscriptional gene silencing
Autore:
Moore, CJ; Sutherland, PW; Forster, RLS; Gardner, RC; MacDiarmid, RM;
Indirizzi:
Hort & Food Res Inst New Zealand Ltd, Auckland, New Zealand Hort & Food Res Inst New Zealand Ltd Auckland New Zealand , New Zealand Univ Auckland, Auckland, New Zealand Univ Auckland Auckland New ZealandUniv Auckland, Auckland, New Zealand
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 8, volume: 14, anno: 2001,
pagine: 939 - 946
SICI:
0894-0282(200108)14:8<939:DGIIPV>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; COAT PROTEIN GENE; MOSAIC-VIRUS; NUCLEOTIDE-SEQUENCE; TRANSGENIC PLANTS; RESISTANCE; INTERFERENCE; SUPPRESSION; TRANSCRIPTS; DEGRADATION;
Keywords:
white clover mosaic virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Gardner, RC Genesis Res & Dev, POB 50, Auckland, New Zealand Genesis Res &Dev POB 50 Auckland New Zealand d, New Zealand
Citazione:
C.J. Moore et al., "Dark green islands in plant virus infection are the result of posttranscriptional gene silencing", MOL PL MICR, 14(8), 2001, pp. 939-946

Abstract

Dark green islands (DGIs) are a common symptom of plants systemically infected with a mosaic virus. DGIs are dusters of green leaf cells that are free of virus but surrounded by yellow, virus-infected tissue. We report here on two lines of evidence showing that DGIs are caused by posttranscriptional gene silencing (PTGS), First, transcripts of a transgene derived from thecoat protein of Tamarillo mosaic potyvirus (TaMV) were reduced in DGIs relative to adjacent yellow tissues when the plants were infected with TaMV. Second, nontransgenic plants coinfected with TaMV and a heterologous virus vector carrying TaMV sequences showed reduced titers of the vector in DGIs compared with surrounding tissues. DGIs also were compared with recovered tissue at the top of transgenic plants because recovery has been shown previously to involve PTGS, Cytological analysis of the cells at the junction between recovered and infected tissue was undertaken. The interface between recovered and infected cells had very similar features to that surrounding DGIs, We conclude that DGIs and recovery are related phenomena, differing in their ability to amplify or transport the silencing signal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:31:05