Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5
Autore:
Fagoaga, C; Rodrigo, I; Conejero, V; Hinarejos, C; Tuset, JJ; Arnau, J; Pina, JA; Navarro, L; Pena, L;
Indirizzi:
Inst Valenciano Invest Agr, Dept Protecc Vegetal & Biotecnol, Valencia 46113, Spain Inst Valenciano Invest Agr Valencia Spain 46113 l, Valencia 46113, Spain Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain Univ Politecn Valencia Valencia Spain 46022 antas, Valencia 46022, Spain
Titolo Testata:
MOLECULAR BREEDING
fascicolo: 2, volume: 7, anno: 2001,
pagine: 175 - 185
SICI:
1380-3743(2001)7:2<175:ITTPCI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHITINASE GENE; FUNGAL PATHOGENS; RESISTANCE; TOBACCO; PROTECTION; INDUCTION; INFESTANS; BLIGHT; GROWTH;
Keywords:
brown rot; disease resistance; foot rot; gummosis; systemic acquired resistance; transgenic trees;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Pena, L Inst Valenciano Invest Agr, Dept Protecc Vegetal & Biotecnol, Apartado Oficial, Valencia 46113, Spain Inst Valenciano Invest Agr Apartado Oficial Valencia Spain 46113
Citazione:
C. Fagoaga et al., "Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5", MOL BREED, 7(2), 2001, pp. 175-185

Abstract

Phytophthora citrophthora is the most widely spread oomycete plant pathogen over all the citrus growing areas and represents one of the major causes of crop losses. Constitutive over-expression of genes encoding proteins involved in plant defence mechanisms to disease is one of the strategies proposed to increase plant tolerance to oomycete and fungal pathogens. P23 (PR-5), a 23-kDa pathogenesis-related protein similar to osmotins, is induced intomato (Lycopersicon esculentum Mill. cv. Rutgers) plants when they are infected with citrus exocortis viroid, and its antifungal activity has been demonstrated in in vitro assays. We have successfully produced transgenic orange (Citrus sinensis L. Obs. cv. Pineapple) plants bearing a chimeric geneconstruct consisting of the cauliflower mosaic virus 35S promoter and the coding region of the tomato pathogenesis-related PR-5. Nine regenerated transgenic lines constitutively expressed the PR protein. They were challengedwith Phytophthora citrophthora using a detached bark assay. A significant reduction in lesion development was consistently observed in one transgenicline in comparison to the control plants. This same line achieved plant survival rates higher than control plants when transgenic trees were inoculated with oomycete cultures. These results provide evidence for the in vivo activity of the tomato PR-5 protein against Phytophthora citrophthora, and suggest that this may be employed as a strategy aimed at engineering Phytophthora disease resistance in citrus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:22:02