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Titolo:
Accelerated production and identification of fertile, homozygous transgenic wheat lines by anther culture
Autore:
Massiah, A; Rong, HL; Brown, S; Laurie, S;
Indirizzi:
IACR Rothamsted, Dept Biochem & Physiol, Harpenden AL5 2JQ, Herts, EnglandIACR Rothamsted Harpenden Herts England AL5 2JQ n AL5 2JQ, Herts, England
Titolo Testata:
MOLECULAR BREEDING
fascicolo: 2, volume: 7, anno: 2001,
pagine: 163 - 173
SICI:
1380-3743(2001)7:2<163:APAIOF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRITICUM-AESTIVUM L; DOUBLED-HAPLOID PLANTS; SINGLE-SEED DESCENT; MICROSPORE CULTURE; HIGH-FREQUENCY; REGENERATION; EXPRESSION; GENES; EMBRYOGENESIS; TRITORDEUM;
Keywords:
anther culture; dihaploid; fertile; homozygous; stable inheritance; transgenic wheat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Massiah, A Hort Res Int, Plant Breeding & Biotechnol Dept, W Malling ME19 6BJ, Kent, England Hort Res Int W Malling Kent England ME19 6BJ BJ, Kent, England
Citazione:
A. Massiah et al., "Accelerated production and identification of fertile, homozygous transgenic wheat lines by anther culture", MOL BREED, 7(2), 2001, pp. 163-173

Abstract

Anther culture has been developed in the winter wheat cultivar Florida to achieve accelerated production and identification of homozygous transgenic lines. With untransformed, seed-derived plants to develop the culture system, it was shown that cold pre-treatment of spikes excised from donor plantsand addition of 2,4-dichlorophenoxyacetic acid together with either kinetin or 6-benzylaminopurine in the callus induction medium improves the antherculture response. The procedure developed allowed production of fertile homozygous lines within 8-9 months, which includes an 8-week vernalisation period. With transgenic wheat plants produced by particle bombardment as donors, we show that the system can be used to produce homozygous transgenics, requiring one generation cycle. Both T-0 tissue culture-derived plants and their T-1 seed-derived descendents serve as suitable donors. We show that an anther culture response comparable to that of untransformed, seed-derivedplants can be achieved with T-0 tissue culture-derived plants. PCR and Southern molecular analyses of anther culture-derived transgenics show that the transgenes are stably inherited; there are no perturbations at the chromosomal level around the sites of transgene integration as a result of in vitro chromosome manipulation during anther culture.

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Documento generato il 05/04/20 alle ore 22:30:00