Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Agrobacterium-mediated transformation of Asparagus officinalis L.: molecular and genetic analysis of transgenic plants
Autore:
Limanton-Grevet, A; Jullien, M;
Indirizzi:
INRA Versailles, UMR INRA INAPG Biol Semences, F-78026 Versailles, France INRA Versailles Versailles France F-78026 es, F-78026 Versailles, France J Marionnet GFA, Lab In Vitro, F-41230 Soings En Sologne, France J Marionnet GFA Soings En Sologne France F-41230 ings En Sologne, France Asparagus bv, Horst, Netherlands Asparagus bv Horst NetherlandsAsparagus bv, Horst, Netherlands
Titolo Testata:
MOLECULAR BREEDING
fascicolo: 2, volume: 7, anno: 2001,
pagine: 141 - 150
SICI:
1380-3743(2001)7:2<141:ATOAOL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-DNA STRUCTURE; INDICA RICE; TUMEFACIENS; BOMBARDMENT; CELLS; BOUNDARIES; EXPRESSION; CULTURES; CALLUS;
Keywords:
Asparagus officinalis L.; Agrobacterium tumefaciens; embryogenic lines; genetic transformation; inheritance of transgenes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Jullien, M INRA Versailles, UMR INRA INAPG Biol Semences, Route St Cyr, F-78026 Versailles, France INRA Versailles Route St Cyr Versailles France F-78026 France
Citazione:
A. Limanton-Grevet e M. Jullien, "Agrobacterium-mediated transformation of Asparagus officinalis L.: molecular and genetic analysis of transgenic plants", MOL BREED, 7(2), 2001, pp. 141-150

Abstract

Four long-term embryogenic lines of Asparagus officinalis were co-culturedwith the hypervirulent Agrobacterium tumefaciens strain AGL1Gin carrying auidA gene and an nptII gene. 233 embryogenic lines showing kanamycin resistance and beta -glucuronidase (GUS) activity were obtained. Transformation frequencies ranged from 0.8 to 12.8 transformants per gram of inoculated somatic embryos, depending on the line. Southern analysis showed that usually1 to 4 T-DNA copies were integrated. Regenerated plants generally exhibited the same insertion pattern as the corresponding transformed embryogenic line. T-1 progeny were obtained from crosses between 6 transformed plants containing 3 or 4 T-DNA copies and untransformed plants. They were analysed for GUS activity and kanamycin resistance. In three progenies, Mendelian 1:1segregations were observed, corresponding to one functional locus in the parent transgenic plants. Southern analysis confirmed that T-DNA copies wereinserted at the same locus. Non-Mendelian segregations were observed in the other three progenies. T-2 progeny also exhibited non-Mendelian segregations. Southern analysis showed that GUS-negative and kanamycin-sensitive plants did not contain any T-DNA, and therefore inactivation of transgene expression could not be responsible for the abnormal segregations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:29:04