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Titolo:
Multiplex PCR for the detection of tetracycline resistant genes
Autore:
Ng, LK; Martin, I; Alfa, M; Mulvey, M;
Indirizzi:
Hlth Canada, Gonococcal Infect Syphilis Sect, Populat & Publ Hlth Branch,Natl Microbiol Lab, Natl Lab Sexually Transmitted Dis, Winnipeg, MB R3E 3R2,Canada Hlth Canada Winnipeg MB Canada R3E 3R2 d Dis, Winnipeg, MB R3E 3R2,Canada Wayne State Univ, Detroit, MI 48202 USA Wayne State Univ Detroit MI USA 48202 e State Univ, Detroit, MI 48202 USA
Titolo Testata:
MOLECULAR AND CELLULAR PROBES
fascicolo: 4, volume: 15, anno: 2001,
pagine: 209 - 215
SICI:
0890-8508(200108)15:4<209:MPFTDO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CAMPYLOBACTER-JEJUNI; NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; CHROMOSOMAL DNA; DETERMINANT; EXPRESSION; STREPTOCOCCUS; PROTECTION; MECHANISMS;
Keywords:
multiplex PCR; tetracycline resistance; MRSA; Salmonella enterica serovar Typhimurium DT104;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ng, LK Hlth Canada, Gonococcal Infect Syphilis Sect, Populat & Publ Hlth Branch,Natl Microbiol Lab, Natl Lab Sexually Transmitted Dis, 1015 ArlingtonSt,Room H2380, Winnipeg, MB R3E 3R2, Canada Hlth Canada 1015 Arlington St,Room H2380 Winnipeg MB Canada R3E 3R2
Citazione:
L.K. Ng et al., "Multiplex PCR for the detection of tetracycline resistant genes", MOL CELL PR, 15(4), 2001, pp. 209-215

Abstract

Specific primer pairs were selected for the PCR amplification of 14 tetracycline resistant genes commonly found in Gram positive and Cram negative organisms. Combinations of primer pairs were used in multiplex PCR reactions to detect specific groups of tel genes as follows; Group I: tet(B), tet(C),tet(D); Group II: tet(A), tet(E), tet(G); Group III. tet(K), tet(L), tet(M), tet(O), tet(S); Group IV: tetA(P), tet(Q), tet(X). To test: the multiplex PCR, Groups I and II were used on 25 clinical isolates of Salmonella enterica serovar Typhimurium DT104. Group III primers were used to investigate 19 clinical isolates of methicillin-resistant Staphylococcus aureus. Multiplex PCR should result in significant savings in terms of labour and cost inanalysis of a large number of strains when compared with using an individual PCR for targeting each gene. It may also be a useful method to differentiate the types of tetracycline resistance when used as an additional markerfor the purpose of outbreak investigation and surveillance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:53:45