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Titolo:
Stepwise progression of centrosome defects associated with local tumor growth and metastatic process of human pancreatic carcinoma cells transplantedorthotopically into nude mice
Autore:
Shono, M; Sato, N; Mizumoto, K; Maehara, N; Nakamura, M; Nagai, E; Tanaka, M;
Indirizzi:
Kyushu Univ, Sch Med Sci, Dept Surg & Oncol, Fukuoka 8128582, Japan KyushuUniv Fukuoka Japan 8128582 t Surg & Oncol, Fukuoka 8128582, Japan
Titolo Testata:
LABORATORY INVESTIGATION
fascicolo: 7, volume: 81, anno: 2001,
pagine: 945 - 952
SICI:
0023-6837(200107)81:7<945:SPOCDA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC INSTABILITY; GAMMA-TUBULIN; CANCER; ADENOCARCINOMA; ABNORMALITIES; ORGANIZATION; PERICENTRIN; ANTIGEN; MODEL; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Mizumoto, K Kyushu Univ, Sch Med Sci, Dept Surg & Oncol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Fukuoka 8128582, Japan
Citazione:
M. Shono et al., "Stepwise progression of centrosome defects associated with local tumor growth and metastatic process of human pancreatic carcinoma cells transplantedorthotopically into nude mice", LAB INV, 81(7), 2001, pp. 945-952

Abstract

Recent evidence indicates that loss of centrosome integrity may be a majorcause of genetic instability underlying various human cancers. The aim of this study was to define the role of centrosome defects during the in vivo tumor progression of pancreatic carcinoma using an orthotopic implantation model. Injection of Suit-2 human pancreatic cancer cells into the pancreataof nude mice reproduced the pattern of local tumor growth and distant metastasis observed in humans. Pancreatic xenografts, peritoneal disseminations, and hepatic metastases were harvested, and tumor cells were examined for centrosomes by immunofluorescence microscopy. Centrosome abnormalities, characterized by increased numbers of centrosomes, were detected in only a small fraction of parental Suit-2 cells in culture, whereas the frequency was markedly increased in cells isolated from the pancreatic xenografts. Abnormal centrosome numbers were found at higher frequencies in metastatic foci than in pancreatic xenografts. A significant positive correlation existed between the fraction of cells with multiple centrosomes and that with multipolar mitotic spindles, suggesting a functional involvement of aberrant centrosomes in spindle disorganization and chromosome missegregation. In addition, the increased frequency of abnormal centrosomes was associated with an enhanced degree of chromosomal instability. These findings suggest a novel model of pancreatic tumor progression whereby a stepwise increase in the magnitude of centrosomal abnormalities confers an increased chance for aberrant mitotic events, thus accelerating genetic instability and causing the tumor to progress to a more advanced stage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:42:31