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Titolo:
Herpesvirus quiescence in neuronal cells IV*: Virus activation induced by pituitary adenylate cyclase-activating polypeptide (PACAP) involves the protein kinase A pathway
Autore:
Danaher, RJ; Savells-Arb, AD; Black, SA; Jacob, RJ; Miller, CS;
Indirizzi:
Univ Kentucky, Coll Dent, Oral Med Sect, Dept Oral Hlth Practice, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 th Practice, Lexington, KY 40536 USA Univ Kentucky, Coll Med, Dept Oral Hlth Practice, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 th Practice, Lexington, KY 40536 USA Univ Kentucky, Coll Dent, Dept Microbiol & Immunol, Markey Canc Ctr, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ey Canc Ctr, Lexington, KY 40536 USA Univ Kentucky, Coll Med, Dept Microbiol & Immunol, Markey Canc Ctr, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ey Canc Ctr, Lexington, KY 40536 USA
Titolo Testata:
JOURNAL OF NEUROVIROLOGY
fascicolo: 2, volume: 7, anno: 2001,
pagine: 163 - 168
SICI:
1355-0284(200104)7:2<163:HQINCI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; NERVE GROWTH-FACTOR; SUPERIOR CERVICAL-GANGLION; DIFFERENTIATED PC12 CELLS; SIMPLEX-VIRUS; NEURITE OUTGROWTH; MESSENGER-RNA; CYCLIC-AMP; SELECTIVE INHIBITOR; TRIGEMINAL GANGLION;
Keywords:
pituitary adenylate cyclase-activating polypeptide (PACAP); neuronal; herpes simplex virus; viral latency; reactivation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, CS Univ Kentucky, Coll Dent, Oral Med Sect, Dept Oral Hlth Practice, MN 118,800 Rose St, Lexington, KY 40536 USA Univ Kentucky MN 118,800 Rose St Lexington KY USA 40536 536 USA
Citazione:
R.J. Danaher et al., "Herpesvirus quiescence in neuronal cells IV*: Virus activation induced by pituitary adenylate cyclase-activating polypeptide (PACAP) involves the protein kinase A pathway", J NEUROVIRO, 7(2), 2001, pp. 163-168

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a naturally occurring peptide found in the central nervous system that plays a role in somatosensory processing and activation of protein kinase A (PKA) and protein kinase C (PKC), Because activation of PKA or PKC results in reactivationof HSV-1 from latently infected embryonic neuronal cells, PACAP was used to evaluate HSV-1 activation from quiescently infected (QIF)-PC12 cells. Ourstudies demonstrate that physiologically relevant concentrations of PACAP38 and PACAP27 induce HSV-1 activation from QIF-PC12 cell cultures in a dose-dependent fashion. PACAP-induced activation of virus was significantly impaired by the PKA-inhibitor, H-89 (20 muM), whereas treatment with the PKC-inhibitor, GF109203X (1 muM), was without affect. Additionally, direct activation of PKA with cAMP analogs, 8-(4-chlorophenylthio)- and dibutyryl-cAMP only partially mimicked the effect of PACAP on virus activation. Taken together, PACAP induced HSV-1 activation from QIF-PC12 cells involves the PKA and possibly cAMP-independent pathways. This report is the first to demonstrate that PACAP induces HSV-1 activation from a quiescent state and that this in vitro cell model is useful for studying early inductive events that lead to virus production from quiescence.

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Documento generato il 08/04/20 alle ore 09:10:41